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Item Inoculação de salmonella enterica subespecie enterica sorovar enteritidis fagotipo 4 em ovos embrionados de duas linhagens de frango de corte(Universidade Federal de Goiás, 2005-09-16) Andradae, Maria Auxiliadora; Stringhini, José Henrique; Brito, Luiz Augusto Batista; Mesquita, Albenones José de; http://lattes.cnpq.br/3180029815183858; Mesquita, Albenones José de; Moraes, Sandra Regina Pires de; Meireles, Marcelo Vasconcelos; Jayme, Valéria de SáTwo experiments were carried out to evaluate the use of quaternary ammonia on Salmonella Enteritidis inoculated in eggshell and its penetration capacities, verify the ability to infect the egg inoculated in eggshell, determine embryo mortality, infect hatched chicks and affect incubation parameters of two broiler lines. A total of 302 and 290 fertile eggs of Ross and ISA Label, respectively, were distributed in six treatments: eggs sanitized with placebo (Treatment 1- PC) quaternary ammonia inoculated with Salmonella Enteritidis (Treatment 2-PI); eggs non-sanitized and inoculated placebo (Treatment 3- NPC) with Salmonella Enteritidis (Treatment 4- NPI); eggs inoculated in allantoidal cavity with placebo (Treatment 5-CAC) or Salmonella Enteritidis (Treatment 6- CAI). Immediately after inoculation, the eggs were hatched and embryo mortality was evaluated after 96, 432 and 528 hours. The qualitative results were analyzed by non-parametric tests of chi-square and Kruskall-Wallis. The incubation parameters were not affected when the pathogen was inoculated in eggshell. It was observed that Salmonella Enteritidis inoculated in allantoidal cavity determined late embryo mortality in fast 17,02% and slow growing 13,04% lines, and eggs inoculated in allantoidal cavity originated chicks with high frequency of intestinal colonization by Salmonella Enteritidis of 76,67% and 26,67% Ross and ISA Label, respective.Item Inoculação de Salmonella enterica subespécie enterica sorovar enteritidis fagotipo 4 em ovos embrionados de duas linhagens de frango de corte(Universidade Federal de Goiás, 2006-09-16) Andrade, Maria Auxiliadora; Mesquita, Albenones José de; http://lattes.cnpq.br/3180029815183858; Mesquita, Albenones José de; Moraes, Sandra Regina Pires de; Meireles, Marcelo Vasconcelos; Leandro, Nadja Susana Mogyca; Jayme, Valéria de SáTwo experiments were carried out to evaluate the use of quaternary ammonia on Salmonella Enteritidis inoculated in eggshell and its penetration capacities, verify the ability to infect the egg inoculated in eggshell, determine embryo mortality, infect hatched chicks and affect incubation parameters of two broiler lines. A total of 302 and 290 fertile eggs of Ross and ISA Label, respectively, were distributed in six treatments: eggs sanitized with placebo (Treatment 1- PC) quaternary ammonia inoculated with Salmonella Enteritidis (Treatment 2-PI); eggs non-sanitized and inoculated placebo (Treatment 3- NPC) with Salmonella Enteritidis (Treatment 4- NPI); eggs inoculated in allantoidal cavity with placebo (Treatment 5-CAC) or Salmonella Enteritidis (Treatment 6- CAI). Immediately after inoculation, the eggs were hatched and embryo mortality was evaluated after 96, 432 and 528 hours. The qualitative results were analyzed by non-parametric tests of chi-square and Kruskall-Wallis. The incubation parameters were not affected when the pathogen was inoculated in eggshell. It was observed that Salmonella Enteritidis inoculated in allantoidal cavity determined late embryo mortality in fast 17,02% and slow growing 13,04% lines, and eggs inoculated in allantoidal cavity originated chicks with high frequency of intestinal colonization by Salmonella Enteritidis of 76,67% and 26,67% Ross and ISA Label, respective.Item Efeitos dos níveis de células somáticas no leite sobre o rendimento e a qualidade do queijo mussarela(Universidade Federal de Goiás, 2007-12-19) COELHO, Karyne Oliveira; OLIVEIRA, Antonio Nonato de; http://lattes.cnpq.br/8261502405023882; LAGE, Moacir Evandro; http://lattes.cnpq.br/2053334263459111; MESQUITA, Albenones José de; http://lattes.cnpq.br/3180029815183858The subclinical mastitis is a mammary gland inflammatory reaction that is characterized by increased levels of somatic cells in the milk. The milk with higher somatic cell counting (SCC) shows alteration in the composition that alters the quality of the milk products. This study aimed to evaluate the effects of the SCC in the milk products in relation to the microbiologic and the physical-chemistry features of the mozzarella cheese. It was chosen cattle with SCC 200.000 cells/mL; SCC >200 a 400.000 cells/mL; SCC >400.000 cells/mL to 750.000 cells/mL and SCC >750.000 cells/mL. The animals did not receive antimicrobial treatment before and on the day of the milk collection. The mozzarella cheese preparation were realized at Planta de Laticínios do Centro de Treinamento da Agência Rural. It was analyzed the milk sub products compositions. The cheese were evaluated after 1, 15 and 30 days of maturation or storage at 7°C for the pH, acidity, humidity, total and soluble protein, fat, total dry extract, defatted, milk acid bacteria count, total and fecal coliforms and psicotrofics microorganism. The experiment was repeated four times in order to demonstrate reproducibility. The results were homogenous for the residues (turkey test) and the variances were compared using a F test, adopting =0,05. The milk with high SCC showed low concentration of protein and the higher nitrogen levels. There was loosing of protein and fat to serum. The cheese elaborated from milk with higher SCC (>750.000cells/mL) showed lower protein level, more humidity and less industrial income and presented lower growth of lactic acid bacteria during the maturation time. Mozzarella cheese prepared with milk containing SCC around 400.000 cells/mL presented increased proteolysis. The results showed here demonstrated that in order to get a good quality mozzarella cheese is necessary the utilization of the milk with somatic cell counts bellow 400.000 cells /mLItem FLUIDOTERAPIA AQUECIDA NO CONTROLE DA HIPOTERMIA EM CADELAS SUBMTIDAS A OVARIOHISTERECTOMIA SOB ANESTESIA INALATÓRIA(Universidade Federal de Goiás, 2008-06-13) ATAYDE, Ingrid Bueno; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; ALVES, Rosangela de Oliveira; http://lattes.cnpq.br/5593911382353878; SILVA, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647Hypothermia is a frequent event in the per-operative period. Usually neglected in Brazil, it may lead to complications such as delay on anesthetic recovery, blood gas impairment, brain and myocardial ischemia and postoperative shivering. Prevention of hypothermia includes aspects from hospital facilities to training the nurse team. Heated fluid infusion is an aid on maintaining body temperature. A recent release of Brazilian industry, the SAF® Fluid Heating System, is a device which maintains warm the fluid to be infused, intended to reduce risks related to per-operative hypothermia. The following study aimed to evaluate clinical and electrocardiographic parameters, blood gas analysis, and serum biochemistry of female dogs submitted to elective ovariohysterectomy and infusion of 0,9% saline solution heated at 37ºC by SAF®. Thirteen female dogs were allocated in two groups: GI control (n=6), and GII treatment (n=7), in an operating room acclimatized at 22ºC. The parameters were evaluated along 90 minutes from pre-anesthetic medication. There were no significant clinical or biochemical variations; however there was group effect on the variables mean arterial pressure, urea, ALT, ALP and hypnosis time. There was no expressive variation on blood gas analysis, although some variables presented significant differences along the moments, but not between the groups. Electrocardiographic significant alterations were not evident, except for P wave, indicating atrial overload in GI. The isolated use of SAF®, on the conditions and degree of hypothermia of this study, was not enough to avoid hypothermia in female dogs submitted to general inhalatory anesthesia.Item PERFIL SANITÁRIO DE BOVINOS DA RAÇA CURRALEIRO FRENTE A ENFERMIDADES DE IMPORTÂNCIA ECONÔMICA(Universidade Federal de Goiás, 2008-06-20) SANTIN, Ana Paula Iglesias; KIPNIS, Ana Paula Junqueira; http://lattes.cnpq.br/1252262903952987; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; BRITO, Wilia Marta Elsner Diederichsen de; http://lattes.cnpq.br/7605775995731168Com o objetivo de ampliar os conhecimentos sobre os aspectos sanitários de bovinos da raça Curraleiro, foi realizado o estudo comparativo entre animais Curraleiros e outras raças bovinas (Guzerá, Nelore, Girolando e Caracu), em relação a enfermidades de importância econômica e epidemiológica para pecuária. Para tanto avaliou-se a ocorrência de eventual diferença no perfil sanitário destes animais através de pesquisa de anticorpos para B. abortus, Leptospira spp, Neospora caninum, Toxoplasma gondii, vírus da leucose enzoótica bovina (BLV), herpesvírus bovino-1(BHV-1), vírus da diarréia viral bovina (BVDV) e realização de reação intradérmica para Mycobacterium bovis. As fêmeas bovinas eram provenientes de propriedades localizadas em Goiás e Tocantins e na região de divisa entre Goiás, Minas Gerais e Bahia (Trijunção). Em relação às doenças bacterianas, nenhum dos animais foi reagente para brucelose e tuberculose. Por outro lado, todas as propriedades estudadas apresentaram animais sororreagentes para Leptospira, N. caninum, T. gondii, BLV, BHV-1 e BVDV. Ao se comparar o Curraleiro com as outras raças separadamente, verificou-se para leptospirose que houve um número significativamente maior de animais sororreagentes da raça Guzerá. Em relação à neosporose os Curraleiros apresentaram um número significativamente maior de bovinos sororreagentes do que a raça Guzerá e Nelore. Resultado semelhante foi observado na toxoplasmose ao se comparar o Curraleiro com o Caracu. Para as demais enfermidades a resposta sorológica foi semelhante entre os Curraleiros e as outras raças. Para leucose enzoótica bovina ainda foram avaliados os níveis de proteínas totais, de frações protéicas, além da contagem de leucócitos e linfócitos, sendo os resultados comparados entre animais reagentes e não reagentes ao BLV, independente da raça. No entanto só foi observada diferença significativa em relação à gamaglobulina que encontrava-se aumentada em bovinos sororreagentes e os linfócitos, que apesar de apresentarem-se dentro dos padrões considerados normais estavam com níveis mais elevados nos animais soropositivosItem Aspectos da resposta imune humoral e celular de bovinos naturalmente infectados com mycobacterium bovis e avaliação de vacina de subunidade protéica para tuberculose em camundongos(Universidade Federal de Goiás, 2008-08-05) SILVA, Ediane Batista da; KIPNIS, André; http://lattes.cnpq.br/4434965360286741; KIPNIS, Ana Paula Junqueira; http://lattes.cnpq.br/1252262903952987Several aspects of the bovine tuberculosis were analyzed in this study. The immunogenicity of recombinant MPT-51 (rMPT-51), Ag-85 and M. bovis-BCG were characterized in an immunosorbent assay, where 208 serum samples from positive intradermal tuberculin test (ITT) animals and 54 serum samples from ITT negative animals where analyzed. M. bovis-BCG and Ag-85 were strongly recognized by antibodies from naturally infected cattle. Additionally, the clinical status of the animals were correlated with the ITT positivity, with the specific production of IL-4 by TCD4 and TCD8 positive lymphocytes, and with nitric oxide (NO) production by macrophages from naturally tuberculosis infected bovine peripheral blood. ITT positive animals showed TCD4+IL4+ cells specific to M. bovis-BCG extract. High background levels of TCD8+IL-4+ lymphocytes were observed in ITT positive animals independent of the stimuli. When cell cultures where stimulated with M. bovis-BCG protein extract, there was no observed difference in NO production between the groups. Naturally tuberculosis infected bovine presented TCD4+IL4+ cells specific for M. bovis- BCG and a preserved NO production. Finnally, the immunogenicity of rMPT-51 use as a proteic sub-unit vaccine was evaluated in BALB/c mice, with two different adjuvants, incomplete Freund and CpG DNA. For this, mice were immunized and challenged with M. tuberculosis. Immunization with rMPT-51 antigen and either adjuvant induced, in the lungs, a migration increase of TCD5+IFN + cells specific for rMPT-51, when compared to controls (P<0.05). rMPT-51 plus CpG DNA presented a better performance among the different vaccination schemes tested, in part due to the ability of stiulate TCD5+IFN + cells and hampering the bacterial load, thus preserving the functional integrity of challenged mice lungsItem ISOLAMENTO DE NEOSPORA CANINUM DE BOVINOS E CARACTERIZAÇÃO BIOLÓGICA DE ISOLADOS EM CAMUNDONGOS BALB/c(Universidade Federal de Goiás, 2008-08-14) MELO, Débora Pereira Garcia; SILVA, Andrea Caetano da; http://lattes.cnpq.br/1432918585717355The objective of this work was to obtain new isolates of N. caninum and characterize the same pathogenically. In Nerópolis (Goiás, Brazil), two Holstein-Friesian male calves, aged four months, positive for indirect immunoflourescence for Neospora with a titre of 1:400 and 1:800 were utilized for the isolation. Two new isolates, NC- Goiás 1 and NC-Goiás 2, were obtained. NC-Goiás 2 was discarded, however, due to fungal contamination. In addition to characterization of isolate NC-Goiás 1, pathogenic characterizations were done for six Spanish isolates in female mice of the lineage Balb/c. Symptomatology and mortality caused by the isolates, parasitaemia, detection of parasite DNA in the brain and lungs were verified, as well as parasite load in the brain throughout the experiment. Further, the development of humoral immune response of the animals was verified. Statistical analysis was carried analysing the effect of isolate and the infection phase. Differences between percentages encountered through nested-PCR were analysed using the Chi-square test. When significant differences were observed (P<0,05), the groups were compared two by two. The difference between parasite loads, optical density values for IgG1, IgG2a and the relation IgG1/IgG2a between the isolates were analysed by the Kruskal-Wallis test. When statistically significant differences were observed (P<0,05), the groups were compared two by two using the Mann-Whitney U test. In blood and lungs, the parasite was detected mainly in the acute phase (P<0,05). The DNA of N. caninum was also detected in the brain mainly in the chronic phase (P<0,05). On comparing parasite loads between isolates, statistically significant differences were observed between them in the chronic phase (P< 0,05). Antibodies of class IgG1 and IgG2a were detected starting from day 16 P.I. and 4 P.I. respectively, increasing gradually and remaining high until day 64 P.I. An analysis of the relation IgG1/IgG2a demonstrated predominance of IgG2a on day 8 e 16 P.I. and highest levels of IgG1 were detected on day 32 and 64 P.I. The isolate NC-Goiás 1 is the first isolate obtained in Goiás and the first obtained from an infected calf, though clinically healthy, reported from South América. These results are the first of a study of the diversity of the protozoon, N. caninum, and indicate that these isolates are of low pathogenicity in mice as compared with other known isolatesItem ASPECTOS DA ECOLOGIA QUÍMICA DE Rhipicephalus sanguineus (Latreille, 1806) (ACARI: IXODIDAE) EM DUAS RAÇAS DE CÃES(Universidade Federal de Goiás, 2008-08-15) LOULY, Carla Cristina Braz; BORGES, Lígia Miranda Ferreira; http://lattes.cnpq.br/2591106734434348This study aimed to identify 2,6-dichlorophenol (2,6-DCP) in Amblyomma cajennense and to evaluate its role in A. cajennense and Rhipicephalus sanguineus courtship. Hexanic extract from attractive females was purified by solid phase extraction and the phenol was identified by the single ion monitoring method using GC/MS. In an olfactometer, the responses of A. cajennense and R. sanguineus males to females, control rubber septa or rubber septa impregnated with 2,6-DCP at 50, 500, and 5000 ng, respectively, were studied. 2,6-DCP was identified in A. cajennense extract and the males oriented themselves toward the concentration of 500 ng. These septa and the females were recognized as copula partners. The septa treated with 2,6-DCP did not attract and were not even recognized by the R. sanguineus males, whereas the females were recognized. Due to the presence of 2,6-DCP in A. cajennense and the results of biological bioassays, it was concluded that this compound acts as an attractant and mounting sex pheromone in this tick, but it does not play any role in R. sanguineus courtship.Item Suplementação de glutamina em dietas iniciais para frangos de corte(Universidade Federal de Goiás, 2008-08-25) LOPES, Karina Ludovico de Almeida Martinez; STRINGHINI, José Henrique; http://lattes.cnpq.br/8505634095383289; CAFÉ, Marcos Barcellos; http://lattes.cnpq.br/9860968235125158; LEANDRO, Nadja Susana Mogyca; http://lattes.cnpq.br/3136809931691012The objective of this study was to evaluate the supplementation of glutamine in the diet on performance, digestibility of nutrients and integrity of the intestinal mucosa of broiler chicks. Were conducted three experiments at the Veterinary School of the University Federal of Goias, using broiler chicks, male, with one day of age. In Experiment I and II was evaluated the supplementation of glutamine for chicks subjected to challenges of different intensities with Eimeria acervulina or Eimeria ssp, and in Experiment III evaluated the effect of supplementation of glutamine on diets containing ingredients of animal origin. The performance was determinated by weight, weight gain, feed intake, feed conversion rate and viability. The digestibility of nutrients was assessed with determination of digestibility coefficients of dry matter, crude protein and ether extract. The intestinal integrity was assessed by the villus height, crypt depth and villus:crypt ratio of the small intestine. The data were submitted to the analysis of variance and for the levels of glutamine was used polynomial regression. In Experiment I after the challenge with Eimeria, were observed better digestibility coefficients for dry matter, crude protein and ether extract with glutamine supplementation on 1.0% (P<0.05). For the results of histomorphometry at 14 days of age it was beneficial effect with the supplementation on 1.0% of glutamine (P<0.05) in the villus height and crypt depth in the duodenum. When challenged, the birds presented better results for villus height and crypt depth in the duodenum at 21 days of age, and in the duodenum and ileum at 28 days of age, when fed 1.0% of glutamine in the ration. In Experiment II the glutamine supplementation did not increase the digestibility coefficient of dry matter and fat on the period of this study. Nevertheless there was significant regression for the digestibility for the second period. On 21 days and 28 days of age the challenged birds showed bigger villus height and crypt depth when the birds was supplemented with 1,0% of glutamine on diet. In Experiment III there was positive quadratic effect (P<0,05) for the crude protein coefficient of digestibility with the increasing levels of glutamine in the ration. The birds belonged to the supplemented groups with glutamine presented better results (P<0,05) for villus height and crypt depth in the duodenum compared to the non-supplemented group. The level of 1,0% of glutamine in the diet presented recovery in the integrity of the small intestine on challenged birds, improving the coefficient of crude protein digestibility of the diet. The utilization of ingredients from animal source in diets for broilers in the initial phase did not harm the performance of chicksItem ATIVIDADES ANGIOGÊNICA, ANTIINFLAMATÓRIA, CICATRIZANTE E ANTIBACTERIANA DO EXTRATO ETANÓLICO E FRAÇÕES DAS FLORES DA Calendula offIcinalis L. CULTIVADAS NO BRASIL(Universidade Federal de Goiás, 2008-09-11) PARENTE, Leila Maria Leal; BRITO, Luiz Augusto Batista; http://lattes.cnpq.br/3212353728321020; PAULA, José Realino de; http://lattes.cnpq.br/3191837532986128; PAULO, Neusa Margarida; http://lattes.cnpq.br/7949273598509043The Calendula officinalis L. is an annual herb, originating in the Mediterranean region, belonging to the Asteraceae family and well known as calendula. Displays orange or yellow flowers, which are attributed medicinal properties, with emphasis on healing activity. In this study, the ethanolic extract of flowers of C. officinalis grown in Brazil was prepared by soaking the cold and fractionated to obtain fractions of dichloromethane and hexane. The angiogenesis activity of the extract and fractions was evaluated using the model of membrane corioalantóide in embryonated chicken eggs. But the healing effect of the extract was evaluated by the model of skin wounds in rats, with assessment of macroscopic and microscopic characteristics. In this model, the ethanol extract of angiogenesis activity in the dermis of rats was also evaluated by immunohistochemistry, and measured the intensity of expression of vascular endothelial growth factor (VEGF). The in vitro antibacterial activity of extract and fractions was evaluated by determining the minimum inhibitory concentration (MIC) using the dilution plate. The ethanol extract and the fractions of calendula showed marked angiogenesis activity, demonstrated in both experimental models used. This activity was not related to the observed increase in intensity of expression of VEGF, which may be related to other pro-angiogenic factors. In the model of skin wounds in rats the ethanolic extract showed anti-inflammatory activity, demonstrated by the decrease in serous exudation of hyperemia, the deposition of fibrin and epidermal hyperplasia, besides the presence of more thin crusts and soaked in the control group. There was an increase in the amount of collagen in granulation tissue and antibacterial activity was observed, mainly on gram-positive bacteria. Still, the C. officinalis served as positive about the process of vascular neoformation and activity in healing skin wounds in rats, and also presented antibacterial activity in vitro. Thus, it is the healing effect attributed to this speciesItem Aspectos epidemiológicos de alterações do aparelho locomotor e uso da arnica montana no tratamento da tendinite experimental em eqüinos(Universidade Federal de Goiás, 2008-09-29) MARANHÃO, Renata de Pino Albuquerque; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; SILVA, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647Item Níveis de lisina e arginina digestíveis, de sódio e número de mongin na ração préinicial de frangos de corte(Universidade Federal de Goiás, 2008-09-29) THON, Mônica Schaitl; LEANDRO, Nadja Susana Mogyca; http://lattes.cnpq.br/3136809931691012; CAFÉ, Marcos Barcellos; http://lattes.cnpq.br/9860968235125158; STRINGHINI, José Henrique; http://lattes.cnpq.br/8505634095383289An experiment was carried out to evaluate performance, development of digestive organs of young broiler chicks (one to twenty-one days of age) fed diets with different levels of digestible lysine in pre-starter diet (1 to 7 days old). A total of 480 day old Cobb 500 male chicks allotted in brooded batteries in a randomized block design with four levels of digestible lysine (1,050, 1,200, 1,350 e 1,500 %) with 10 replicates of 12 chicks each. Weight gain, feed intake, feed: gain ratio and development of digestive organs were evaluated. For statistical analysis the program SAEG developed by the Federal University of Viçosa (UFV) was used. Analysis of polynomial regression was done for digestile lysine levels. No significant effect on broiler performance. Digestible lysine recommended for male broilers was 1,050% in pre-starter diet (1 to 7 days old).Item Prevalência de dentes tratados endodonticamente em uma população de adultos brasileiros(Universidade Federal de Goiás, 2008-11-11) HOLLANDA, Augusto César Braz; ESTRELA, Carlos; http://lattes.cnpq.br/3611967334176683This cross-sectional study examined the prevalence of endodontically treated teeth in 1,401 Brazilian adults. Panoramic radiographs were selected at the Radiological Center of Orofacial Images (CRIOF, Cuiabá, MT, Brazil) between August 2002 and September 2007. Three independent endodontists discussed interpretation criteria and classified specimens according to the following data: presence of root canal treatment, which was defined as partially or completely filled canal space, regardless of whether filling ended at the radiographic apex or not; presence of intracanal post; and associated apical periodontitis. Odds ratio, logistic regression and a chi-square test were used for statistical analyses. Significance level was set at p<0.05. Of 29,467 teeth evaluated, 6,313 (21.4%) were treated endodontically. Endodontic treatment was most frequent in maxillary premolars and molars, whereas mandibular incisors showed the lowest prevalence. Most endodontically treated teeth were found in people aged 46 to 60 years (47.6%, p<0.001) and the prevalence increased with age in this age range. Females (61.9%, p<0.001) showed a higher prevalence of teeth with root fillings than males. The present study found a higher prevalence of endodontically treated teeth in a Brazilian adult population compared to the prevalence reported in epidemiological studies conducted in other countries.Item PERFIL SOCIOECONÔMICO E MICROBIOLÓGICO DE MANIPULADORES E QUALIDADE DE OVOS DE GRANJAS DE PRODUÇÃO COMERCIAL. Influência da Contaminação Experimental por Pseudomonas aeruginosa sobre a Qualidade de Ovos Não-Lavados e Lavados(Universidade Federal de Goiás, 2008-12-05) STRINGHINI, Maria Luiza Ferreira; MESQUITA, Albenones José de; http://lattes.cnpq.br/3180029815183858; ANDRADE, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; LEANDRO, Nadja Susana Mogyca; http://lattes.cnpq.br/3136809931691012This study was developed in order to study the behavior of Pseudomonas aeruginosa inoculated on shell of washed and unwashed eggs. It was evaluated the socioeconomic and microbiological profiles of hands, nasal cavity and oropharynx of 32 volunteers staff from four commercial poultry farms located in the metropolitan region of Goiânia-GO. It was found that 56% of the workers are males and that the most common age group (47%) is between 18 and 23 years. Approximately 41% (13/32) had 6th to 9th grade of elementary school and 44% had monthly family income between two and three minimum salaries. Most microorganisms isolated and identified among the workers belong to the natural microflora of them. However, Escherichia coli was isolated from the hands of 12.5% individuals before starting day of work indicating contamination of fecal origin. Deteriorated microorganisms for eggs also were identified in the handlers as Pseudomonas spp. and Enterobacter spp. Among the Gram-positive bacteria identified detached Staphylococcus coagulase positive that can pose dangers to health of consumers. After this initial research, it was evaluated the bacteriological quality of 576 washed and 132 unwashed eggs of Dekalb White hens obtained in four commercial poultry farms located in the metropolitan area of Goiânia, collected half in the classification hall, and the other half, in the facilities. Counts of mesophilic and positive Staphylococcus coagulase and Most Probable Number (MPN) of total and fecal coliforms in shells and internal content of eggs and Salmonella spp. research in eggshells were made. It was concluded that washed eggs from commercial egg farms had better eggshell bacteriological quality than unwashed eggs but the washing process adopted must be made appropriate in order to avoid contamination. In the following experiment aimed to verify the quality of commercial unwashed eggs submitted to experimental contamination by Pseudomonas aeruginosa. Were contaminated, by handling, with 3.0 x 102 and 6.0 x 105 colony-forming units (CFUs) of Pseudomonas aeruginosa/mL of solution, 576 unwashed eggs without cracks, classified as large, laying hens with 30 to 40 weeks of age. After contamination, the eggs were stored at 5oC and 28oC for 30 days. Every 10 days were carried out analyses of physical quality of eggs (weight of the egg, specific gravity, shell thickness, percentage of yolk, albumen and shell, Haugh unit, rates of yolk and albumen) and pH and counting of the contaminated bacteria in shell and contents of the eggs. During storage, there was control of bacterial multiplication in the shell of the eggs kept at 5oC (p<0,05). However, in content, the cooling controlled the growth of bacteria only in eggs contaminated with initial higher inoculum. It was concluded that the cooling maintains the internal quality of the eggs, even when contaminated and also retains the hygienic-sanitary characteristics of the product. In the last experiment aimed to check the quality of commercial washed eggs inoculated in the shell with Pseudomonas aeruginosa, stored at 5oC and 25oC. It was used 576 eggs, without cracks, classified as large, from laying hens with 30 to 40 weeks of age, line Dekalb White. The experimental design was completely randomized in a 3 x 2 factorial (contamination and temperature storage) with 12 repetitions for physical quality and pH and four repetitions for microbiological variables. The eggs were contaminated by handling, with 7.8 x 102 and 6.0 x 105 colony-forming units (CFUs) of Pseudomonas aeruginosa/mL of solution and stored at 5oC and 25oC for 30 days. Every ten days it was carried out analyses of physical quality of eggs, similar to the previous experiment, pH and counts of Pseudomonas aeruginosa in shell and contents of the eggs. The eggs stored at 5oC showed better internal quality (p<0,05) and lower bacterial counts in shell and contents of the eggs (p<0,05) regardless of the initial concentration of inoculum. It was concluded that the cooling provides better internal quality, physical, chemical and bacteriological of eggs, during 30 days of storage.Item EFEITO DO GRUPO GENÉTICO E PESO DE ABATE NAS CARACTERÍSTICAS DA CARCAÇA E QUALIDADE DA CARNE DE CORDEIROS CONFINADOS(Universidade Federal de Goiás, 2008-12-15) LANDIM, Aline Vieira; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; LOUVANDINI, Helder; http://lattes.cnpq.br/3996385621553357; PIMENTEL, Concepta Margaret Mcmanus; http://lattes.cnpq.br/6052239712915301Sheep rearing is becoming an important economic activity, where it is necessary to establish the ideal slaughter weight per genetic group and sex to acheive standardized carcasses which meet customer needs in terms of product quality.This experiment evaluated the effect of genetic group and slaughter weight on carcass traits and meat quality in lambs. Twenty four Santa Ines (SI), 24 ½ Ile de France x ½ Santa Inês (ILE x SI) and 8 ½ Texel x ½ Santa Inês (TE x SI), slaughtered at different weights (30, 35, 40 and 45 kg ) were used.Eye rib area and fat thickness were measured in vivo and on the carcass. After a 16 hour food and water fast the animals were slaughtered conventionally and the carcasses placed in a cold chamber at 4ºC/24h. pH was measured at slaughter and after 24h as were subjective measurements on the Longissimus dorsi. A section of the rib region from the 11th to 13th ribs of the left side of the carcass was used for tissue and fractional composition, physic-chemical parameters and fatty acid composition. The experiment was in a 3 x 2 x 4 factorial design. For physic-chemical and fatty acids a 3 x 4 factorial and analysed using CORR (Correlation), REG (Regression) and Duncan (5%) means test in SAS ®. Genetic group did not influence (P>0.05) means for rib eye area or fat thickness measured by ultrasound or paquimeter. These measurements increased with slaughter weight. Tissue composition was influenced by genetic group and slaughter weight (P<0.05). The Texel x Santa Inês had most muscle (63.75 g), fat (53.75 g), bone (29.37 g) and edible portion (78.75%) at 35 and 45 kg . Slaughter weight centesimal composition, with an increase in total lipids as weight increased. No significant differences were found for final pH between genetic groups and slaughter weights. Genetic group did not influence colour (3.32), shear force (3.57 kgf) or loss in cooking (24.05%) of the Longissimus dorsi, but slaughter weight affected these, causing stronger colour, tougher meat and greater loss in cooking as slaughter weight increased. Significant differences were found in the sensorial traits of the meat with different genetic groups and slaughter weights. The fatty acids found included oleic (43%); palmitic (22%) and estearico (18%). Higher quantities of desirable fatty acids were found in the Texel x Santa Inês (66.78%) group. Slaughter weight caused a significant difference (P<0.05) in the fatty acid profile of saturated and polyunsaturated fats.Item INFLUÊNCIA DOS TIPOS DE ORDENHA, TRANSPORTE E TEMPO DE ARMAZENAMENTO NA QUALIDADE DO LEITE CRU REFRIGERADO DA REGIÃO SUDOESTE DO ESTADO DE GOIÁS(Universidade Federal de Goiás, 2008-12-19) SILVA, Marco Antônio Pereira da; MOURA, Celso José de; http://lattes.cnpq.br/6006558807438442; PRADO, Cristiano Sales; http://lattes.cnpq.br/1661902818272650; NICOLAU, Edmar Soares; http://lattes.cnpq.br/9601723963736071The objective of the research were evaluate cooled raw milk gotten in dairy properties of the Southwest Goiano in the periods rainy and dry of 2008. The samples were collected of individual producers where the storage in bulk tanks were carried for until 72 hours, with 24-hour intervals. Somatic cells count, total bacterial count and centesimal composition were carried in the Laboratório de Qualidade do Leite of the Centro de Pesquisa em Alimentos of the Escola de Veterinária of the Universidade Federal de Goiás. The microbiological analysis and titratable acidity were carried in the Laboratories of the Unidade de Agroindústria of the Instituto Federal de Educação, Ciência e Tecnologia Goiano Campus Rio Verde GO. Data were submitted to the variance analysis and the analyzed factors were: period, type of milking and storage time, in entirely casualized delineation and factorial arrangement 2 x 2 x 4. The comparison of period and type of milking was carried through by means of test F of the variance analysis. The storage time was analyzed by means of regression models. Software SISVAR was used for analysis. The results of the physical-chemical composition were in accordance with the legislation. The total bacterial count of cooled raw milk to the 24 hours of storage was above of the limit of the legislation. The count of psychrotrophic, psychrotrophic proteolytic and Pseudomonas spp., was bigger in the rainy period.Item SISTEMA DE BARREIRA COM FILME DE HIDROGEL DE POLI(2-HIDROXIETIL METACRILATO) NA PREVENÇÃO DE ADERÊNCIAS PERITONIAIS : ESTUDO EXPERIMENTAL EM RATAS E CADELAS(Universidade Federal de Goiás, 2009-01-16) SILVA, Marcelo Seixo de Brito e; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; PAULO, Neusa Margarida; http://lattes.cnpq.br/7949273598509043Synthetic materials are the leading choice for the correction of defects in abdominal wall, but the formation of adhesions are a problem encountered when placing implants in contact with the abdominal viscera. With In order to avoid its formation, various materials and mechanisms are used in an attempt to prevent contact with peritoneal surfaces of the prostheses. In this sense, we investigated the possibility of the hydrogel poly (2-hydroxyethyl methacrylate) (polyHEMA) coating a face of polypropylene mesh and thereby preventing peritoneal adhesion in dogs and rats, and, in the latter species, also performed a histological evaluation of implanted materials. For this, an abdominal defect was created in the rectus abdominis of 16 bitches were divided into two groups of eight animals and the defect was corrected with screen polypropylene and polypropylene mesh coated with hydrogel, respectively. The presence of adhesions was evaluated after 20 days by laparoscopy. As to rats, the surgical procedure was similar and the evaluations were performed at 15 and 30 days, with euthanasia and collection of fragments for histology with picrosirius red staining and HE The hydrogel coating the polyHEMA polypropylene mesh implanted in the abdominal wall prevented completely visceral adhesions and omentum remaining on the surface the screen when compared to polypropylene mesh in dogs and isolated rats. Was found greater amount of adhesions in the suture zone in the group a mesh coated with polyHEMA. The physical structure of the biomaterial interfered the organization and persistence of tissue response, but not in the same type.Item Caracterização molecular e isolamentode clostrídios psicrofílicos e psicrotróficos associados à deterioração de carnes refrigeradas embaladas a vácuo(Universidade Federal de Goiás, 2009-02-20) BUENO, Cláudia Peixoto; PRADO, Cristiano Sales; http://lattes.cnpq.br/1661902818272650; MESQUITA, Albenones José de; http://lattes.cnpq.br/3180029815183858The deterioration of vacuum packed refrigerated meat accompanied by large gas production - a phenomenon called blown pack - is considered a major cause of economic losses of the meat industry in several regions of Brazil and the world. Several psychrophilic and psychrotrophic microorganisms may be involved, especially species of Clostridium. The objective of the present study was to perform the molecular characterization, through the use of the PCR technique, and the molecular isolation by conventional bacteriology, of the main microorganisms that cause blown pack in refrigerated meat from Brazil, New Zealand and the United Kingdom. Thus, typing techniques were used to differentiate the species and subspecies involved in this type of deterioration. Tirty-six samples of Brazilian blown pack meat, 6 samples from the UK and 12 experimental blown pack samples of venison from the North Island of New Zealand were analyzed. Three pairs of primers, the RFP / RRP, the 16SEF/16SER and the pair EISRF / EISRR were used for C. estertheticum estertheticum and Clostridium estertheticum like, and one for C. gasigenes (16DBF/16DBR). The samples with the PCR results were sent to a microbiology laboratory for conventional isolation of Clostridium estertheticum. It was concluded that Clostridium estertheticum estertheticum is responsible for the deterioration of meat and hence the blown pack in the UK. Samples of blown pack Brazilian meat have Clostridium estertheticum like as primary causal agent. The typing was carried out in isolates and strains - donated by Mirinz Center / Ruakura Agresearch / Hamilton / New Zealand - together with two isolates from Brazil, involved in this type of deterioration. The selected techniques AFLP and RFLP - PCR were able to distinguish species and subspecies of psychrophilic and psychrotrophic clostridia. However, the AFLP showed the highest discriminatory power, being able to distinguish 100% of the species - C. estertheticum, C. frigoris, C. bowmani, C. lacusfryxellense and C. psychrophylum - and also the subspecies C. estertheticum estertheticum, C. estertheticum laramiense, C. estertheticum like k21 and k24. Through the technique of RFLP, it was possible to differentiate the species of clostridia psychrotrophic, psichrophilic and also the subspecies C. estertheticum estertheticum and Clostridium estertheticum like, along with the use of four restriction endonucleases - AluI, CfoI, TaqI and HaeIII. The HaeIII provided greater variety of fragments and the ability to differentiate the species of clostridia psychrophilic and psychrotrophic, whereas TaqI was the only enzyme capable of differentiating the subspecies of C. estertheticum estertheticum and C. estertheticum laramiense of C. estertheticum like. The Brazilian samples isolated fit into the group of Clostridium estertheticum like, although there is no confirmation of the absence of Clostridium estertheticum estertheticum in the country.Item EFEITOS DA INCLUSÃO DE ÁCIDOS GRAXOS INSATURADOS NO SUPLEMENTO DE VACAS PRIMÍPARAS ZEBUÍNAS SOBRE O DESEMPENHO PRODUTIVO E REPRODUTIVO(Universidade Federal de Goiás, 2009-02-26) GODOY, Marcelo Marcondes de; PADUA, João Teodoro; http://lattes.cnpq.br/0308044304591375Were used primiparous cows, zebuine, calved than received in the postpartum period, low intake isoenergetic supplements, in pasture of Brachiaria brizantha cv. Marandu, with twenty cows per treatment in a randomized design, where FA: the low intake supplement (LIS) with inclusion of protected fat, CO: LIS with the inclusion of meal corn and MS: only mineral supplement. Evaluated the body weight of cows (BW) of calves (BWC) and body condition score (BCS) of cows inthe postpartum period, at every 28 days, effect of sex of calf in the BW medium (BWm) and in the BCS medium (BCSm) of cows, the average daily weight gain of cows (ADWG) and calves (ADWGC) and pregnancy rate (PR). The supplementation increased the BW of the cows in FA and CO without difference between them (P> 0.05), already the MI did not differ from SM de108 to 136 days postpartum (P> 0.05). The BWC of FA and CO did not differ (P> 0.05) among them and were higher (P <0.05) than the MS 108 until 192 days postpartum, and heavier at weaning. The cows of FA had BCS higher (P <0.05) than the other treatments from 80 to 192 days postpartum. The ADWG of FA and CO did not differ among them (P> 0.05) and were higher than SM (P <0.05). The ADWGC of FA and CO were similar (P> 0.06), and higher than SM (P <0.06). Cows that nursing males had lower BWm and BCSm that those nursing females (P <0.05). The cows of FA had higher PR (P <0.01)Item Análise multivariada de características que influenciam a tolerância ao calor em equinos, ovinos e bovinos(Universidade Federal de Goiás, 2009-03-27) CASTANHEIRA, Marlos; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; PIMENTEL, Concepta Margaret Mcmanus; http://lattes.cnpq.br/6052239712915301Heat stress is a limiting factor in animal production in the tropics. The physical, physiological and hematological are often used to evaluate the adaptation of animals to heat. The objective was to perform multivariate analysis of physiological characteristic "tolerance to heat, horses, sheep and cattle in the Federal District, to determine whether measured characteristics were able to separate groups of animals and determine the most important variables in differentiating groups in the adaptation of animal heat. A total of 40 horses, 50 sheep and 90 cattle, all adults. The quantitative characters were subjected to multivariate statistical tests of cluster analysis and canonical discriminant. Analyses were performed in the Statistical Analysis System - SAS ® procedures using cluster stepdisc, cancorr and discrim. The dendrogram was able to separate and demonstrate the genetic distance between groups of animals analyzed. The canonical analysis separated the individuals into groups. The discriminant analysis identified the variables that are most important in adapting these to the heat in the Federal District, the conditions were in the polls.