Mapeamento de QTLs para teor de proteína em feijoeirocomum (Phaseolus vulgaris L.)
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2006-12-15
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Universidade Federal de Goiás
Resumo
The common bean besides being one of the basic meals of brazilian´s
population, it is one of the main products that provide protein in the nutritional
diet from the society share which is economically less favorable. The
identification of molecular markers linked to controlling genes of the protein
content in common beans (Phaseolus vulgaris L.) is a very important tool to
help breeding programs, raising the efficiency and agility. This way, this work
was made with two main goals: a) to map SSR and RAPD markers linked to loci
(QTLs) that control protein content in two generations of a segregating
population of common beans and b) to compare detection procedures of
markers linked to QTLs using the ANOVA method and the process of interval
mapping. For that reason, 94 families were taken from the F2 generation and 90
families from the F2:3 generation derived from the cross of genitors CNFC 7812
e CNFC 8056. Results indicated that there is the possibility of identifying
molecular markers related to protein content in common beans, utilizing both
detection procedures. The ANOVA method identified a greater number of QTLslinked
markers than the process of interval mapping in both generations. There
was coincidence between the identified loci obtained with the two methods for
each generation. Loci that were associated with protein content were different
for the F2 and F2:3 generations. However, there was a stable detection of a
genomic region of the linkage group 4, indicating a possible role of this region of
the common bean genome in the control of seed protein content. The proportion
of the trait s phenotypic variation explained by QTLs varied from 5,5% to 9,5%,
considering both generation.
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LEÃO, Ariane Castro Mendes. Mapping QTLs for protein content in common beans
(Phaseolus vulgaris L.). 2006. 111 f. Dissertação (Mestrado em Ciências Biolóicas) - Universidade Federal de Goiás, Goiânia, 2006.