Estudo do potencial de remoção de substâncias húmicas na água com aplicação de enzimas fúngicas
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Data
2011-09-02
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Universidade Federal de Goiás
Resumo
The humic substance (SHs), organic matter in different stages of degradation are formed
during the decomposition of plant and animal residues in the environment, it is
important to remove them, as they can give undesirable characteristics to the water
distributed. The use of microorganisms such as fungi, for this removal are extremely
important, since their role in sanitation is to act on processing organic waste, where they
function as recyclers of matter in different ecosystems. This work aimed to study the
production of phenol oxidase enzymes, peroxidases white decomposition by fungi
(Trametes villosa and Pycnoporus sanguineus) for the removal of HSs in the water. The
work was divided into parts: extracted, purified and characterized the humic and fulvic
acids, was placed just after the HSs in water and we used the fungal enzymes for their
removal, tests were also performed in the Jar Test. Analysis of the composition of HSs
(characterization) and verification of degradation was performed by spectrophotometry
in the ultraviolet and visible region, and infrared spectroscopy, by determining the
enzyme production. The extraction method used was effective in the separation of
humic and fulvic acids, the spectra of extracted humic acids showed carboxylic groups,
alkyl, aromatic, alcohol, phenol and carbohydrates. The predominant enzyme in all
fungal species used was laccase (Lcc); analyzed samples of Trametes villosa showed the
largest decrease in absorbance at several wavelengths in the ultraviolet / visible (UVVis)
spectra, an indirect measure of degradation. The samples containing the fungus
Trametes villosa were also those that had a higher laccase enzyme production in most
trials, reaching a production of 3.48 mL-1 with humic acid from Sigma-Aldrich® brand
and 2.61 U.mL-1 for humic acid extracted and getting a percentage rate of removal of
56% and 42% respectively of these acids. In tests with fulvic acid and fungi at different
pHs (3.0, 5.0 and 7.0), all has decreased range of UV/Vis with percentage removal rates
of 92%. T villosa showed the higher rates of adsorption evidenced byUV-Vis spectrum,
albeit to a lesser extent than those treated withenzymes, their highest percentage
of adsorption was 86%. Fungi in this study have great potential for degradation and
adsorption of humic substances present in water.
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CAMPOS, Rykelly Faria. Study of potential removal of humic substances in the water with application fungal enzymes. 2011. 135 f. Dissertação (Mestrado em Engenharias) - Universidade Federal de Goiás, Goiânia, 2011.