Salmonella sp. em excretas, carcaças e ovos de Gallus gallus comercializados em feiras de Goiânia, Goiás
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2019-12-20
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Universidade Federal de Goiás
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The production and way of selling free-range chickens in fairs are important factors in the transmission of infections such as salmonellosis to humans. This work was carried out with the aim of investigating the occurrence of Salmonella enterica in poultry products sold at free fairs in Goiânia from which 100 samples of chicken carcasses, 100 samples of excretes and 50 dozen eggs were collected. In this study were used bacteriological research, serotyping and determination of antimicrobial resistance profile, and analysis of the genetic profile of the isolates. There was isolation in six excrete samples; five in the carcasses and three in the eggs. Regarding serotyping, there were identified in the carcass Salmonella Cerro, Salmonella Panama, Salmonella Rugosa, Salmonella Corvallis, besides the antigenic samples O:4,5; O:3,10 in the samples of excretes were identified Salmonella Typhimurium, Salmonella Agona, Salmonella Rugosa, Salmonella Anatun and a rough strain; and Salmonella Corvallis in the egg samples. In the analysis of the Resistance Profile 12 antibiotics were tested. For the isolates from the carcasses two did not present resistance to any antibiotics, two showed resistance to two antibiotics, one to just one antibiotic and another one presented multiresistance to drugs. The isolates from excretes three were sensitive to all antibiotics, one isolated to only one antibiotic and one with multiresistance (three antibiotics), while the three isolates from the eggs did not present resistance to any of the antibiotics tested. The result of polymerase chain reaction – PCR, besides confirming the presence of Salmonella in the positive samples from conventional bacteriology also identified the presence in three excrete samples, two carcasses and three of eggs. In addition, PCR also detected the presence of the blaTEM gene in four samples, the qnrS gene in five samples and aadA2 in any of the samples initially positive for Salmonella spp. Using Electrophoresis in Pulsed Field - PFGE, it was possible to identify that four Salmonella Corvallis isolates are part of the same clonal group as well as the two of S. Anatum, while the other identified serotypes belong to different clonal groups among themselves and those previously mentioned. From these results, it is infered that the system of free-range chicken productions, represents an important role in the epidemiology of salmonellosis, either as maintainer or as a disseminator of several Salmonella Enterica serovars, representing a concern for industrial poultry farming and public health.
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CARDOZO, S.P. Salmonella sp. em excretas, carcaças e ovos de Gallus gallus comercializados em feiras de Goiânia, Goiás. 2019. 81 f. Tese (Doutorado em Ciência Animal) - Universidade Federal de Goiás, Goiânia, 2019.