Métodos diagnósticos para o vírus mayaro: revisão sistemática e avaliação molecular em pacientes arbovirose like em unidade municipal de sáude de Goiânia-Goiás
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2020-02-17
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Universidade Federal de Goiás
Resumo
Diseases caused by viruses are considered an often neglected public health problem that
affects thousands of people worldwide, every year. Symptoms like fever, arthralgia and
rash. classify patients as syndromic dengue or arbovirus like. The differential diagnosis of
arbovoresis is by serological and molecular techniques. The present study aimed to
evaluate diagnostic techniques in studies published in the literature in order to develop a
Systematic Literature Review on the detection of the Mayaro virus that allows health
professionals to carry out an efficient laboratory diagnosis, as well as the application of
molecular tests for the diagnosis of MAYV. The available literature was evaluated by
systematic review, focusing on the diagnosis of the Mayaro virus in humans and its
methodologies. The systematic review was carried out through an exploratory study in the
databases: Scielo (Scientific Eletronic Library Online), Medline (Medical Literature
Analysis and Retrieval System Online), Scopus database, PubMed, LILACS from June 8,
2019 to 02 November 2019, selecting scientific articles according to the criteria of the
PRISMA (2009) recommendation. The data showed that the circulation of the Mayaro
virus in urban regions is already well established worldwide and that the misdiagnosis is
frequent due to similar symptoms with other arboviruses. There is a wide use of varied
methods over time in the diagnosis for this arbovirus, requiring further research to
implement a standardized, sensitive and specific method. In a second stage of the
research, between May to August 2017 and January to June 2018, 452 samples of
patients with syndromic dengue symptoms (arboviruses like) were screened. The
samples were sent to the Molecular Genetics and Cytogenetics Laboratory of the Federal
University of Goiás, where they were subjected to molecular screening by Polymerase
Chain Reaction followed by Reverse Transcriptase (RT-PCR) reaction. All positive cases
for the first PCR were confirmed on RT-qPCR for viral detection. Thirteen positive
samples went through both tests and thus were subjected to Sanger sequencing. In this
experimental study, 17 samples were positive for the Mayaro virus infection (RT-PCR and
RT-qPCR), and co-infections with the Dengue virus were also found by molecular
screening carried out in parallel for the Dengue virus by RT-PCR and RT-qPCR. The
positive samples for MAYV / DENV were analyzed phylogenetically, suggesting
circulation of Dengue serotype 2 and a Peruvian strain of Mayaro Virus (strain IQ 4235) in
Goiânia.
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ABRANTES, Gabrielly Regis. Métodos diagnósticos para o vírus mayaro: revisão sistemática e avaliação molecular em pacientes arbovirose like em unidade municipal de sáude de Goiânia-Goiás. 2020. 107 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2020.