Análise topográfica e histológica de enxertos de mucosa ceratinizada removidos com mucótomo ou bisturi convencional: estudo ex-vivo em mandíbula suína.
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2022-09-14
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Universidade Federal de Goiás
Resumo
To evaluate the surface and tissue quality of EMC obtained by the technique with
conventional scalpel and mucotome. Methods: This is an experimental in vitro/ex vivo
study. Six swine hemi-mandibles that had a favorable keratinized tissue condition were
used for specimen removal in the anterior region of the mandible. Specimens were
obtained using mucotome and conventional scalpel. For the purpose of greater
precision, the procedure for removing specimens from the jaws was performed by a
specialized and previously trained dental surgeon, offering the same conditions for
graft removal by both techniques. random drawing to determine the technique to be
performed for tissue removal. The specimens were prepared according to the protocols
pre-established by the laboratories and then they were taken for analysis in optical
microscopy to evaluate the epithelium and connective tissue and in scanning electron
microscopy for analysis of topography and 3D profilometry. Results: Two areas were
analyzed per sample with dimensions of 1920 x 2560 μm. It was possible to notice the
presence of keratinized stratified squamous epithelial tissue and dense connective
tissue modeled on the tissue slides removed with mucotome and scalpel. In the tissues
removed with mucotome, there is a linearity in the tissue base and in the thickness of
the tissue as a whole, presence of submucosa, fibrous connective tissue that is ideal
for the success of the graft Differences in the surface of the samples removed by the
two techniques were observed when to compare the images obtained through the two
types of microscopy. Conclusion: Keratinized mucosa grafts when removed by the
mucotome technique present greater uniformity and lower presence of undesirable
cells compared to the scalpel technique, favoring a greater chance of success in
surgical procedures for soft tissue grafting.
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SANTOS, Kleber Vinícius Rodrigues dos. Análise topográfica e histológica de enxertos de mucosa ceratinizada removidos com mucótomo ou bisturi convencional: estudo ex-vivo em mandíbula suína. 2022. 109 f. Dissertação (Mestrado em Odontologia ) - Universidade Federal de Goiás, Goiânia, 2022.