Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum
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2014-10-23
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Universidade Federal de Goiás
Resumo
The genus Trichoderma is represented by non-pathogenic soil fungi that have been
studied by act as biological control agents against fungal pathogens, such as
Sclerotinia sclerotiorum, the fungus that causes white mold, besides that, reduce
environment and human health risks by being able to replace agrochemicals.
Proteomic strategies with MS techniques have been important tools in studies of
patterns identification for protein expression in different growth conditions. This work
aims to development new strategies that enable the detection and identification of
proteins secreted by Trichoderma asperellum (TR356) and Sclerotinia sclerotiorum
when inoculated together and separated. The results obtained by MALDI / TOF
analysis allowed the identification of a β-1.3--glucanosiltransferase and α-1.2-Dmannosidase,
demonstrating the possibility of proteins identification for better
comprehension about interaction between these organisms. It was therefore possible
to identify the proteins through strategies used, but further analysis are required in
order to elucidate the function and interaction of proteins secreted by the fungus
Trichoderma asperellum TR356 against S. sclerotiorum.
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RODRIGUES, A. R. Análise do secretoma de isolados do fungo Trichoderma asperellum (TR356) em resposta ao fungo fitopatogênico Sclerotinia sclerotiorum. 2014.86 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2014.