N-glicosilação de 5-(1-(3-fluorofenil)-1H-pirazol-4-il)-1H-tetrazol catalisada por células fúngicas livres e imobilizadas de Cunninghamella echinulata ATCC 9244
Nenhuma Miniatura disponível
Data
2015-02-12
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Biotransformations are powerful tools for optimization of active compounds, diversification and structural modifications, in addition to handling non-functionalized compounds unfeasible by chemical conventional methods. The versatility of microbial systems, in this context, and the numerous entitlements of filamentous fungi have leveraged the use of biotransformations with microorganisms. Glycosylations ate one of the most important and commom modification processes and the use of biocatalysts have set a favorable strategy to this type of reaction. Cunninghamella species, for its enzimatic arsenal, are widely applied, especially for enabling the production of novel drug and cell immobilization hás been evidenced as a tool to express its enzimatic activity.Considering the relevance of amino sugars and their enormous potential in various therapies, the aim of this study was to produce 5-(1-(3-fluorophenyl)-1H-pyrazol-4-yl)-1H-tetrazole (LQFM 021) derivatives, which contains tetrazole and pyrazole rings, by biotransformation with free and immobilized fungi cells of Cunninghamella echinulata ATCC 9244. Therefore, CLAE methodologies were developed for the analysis of biotransformation kinectics and characterization techniques employed (Nuclear Magnetic Resonance (NMR) and Mass Spectrometry with Ion cyclotron Resonance (FTICR-MS)) of the product. 96-hour incubations were proceeded with free cell Cunninghamella echinulata ATCC 9244, in the PDSM culture medium and immobilized cells in three separate experiments: biofilm incubated in PBS buffer, biofilm incubated in PDSM medium culture and reuse of biofilms, where one derivative was obtained. The structural characterization for the purified derivative obtained in sufficient quantities is a 1-(5-(1-(3-fluorophenyl)-1H-pyrazol-4-yl)-2H-tetrazol-2-yl)pentane-1,2,3,4,5-pentaol.
Descrição
Palavras-chave
Citação
SOUZA, P. L. M. N-glicosilação de 5-(1-(3-fluorofenil)-1H-pirazol-4-il)-1H-tetrazol catalisada por células fúngicas livres e imobilizadas de Cunninghamella echinulata ATCC 9244. 2015. 81 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2015.