Detecção de Magnaporthe oryzae em sementes de arroz por meio da técnica LAMP (amplificação isotérmica de ácidos nucleicos)
Nenhuma Miniatura disponível
Data
2016-02-16
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Considered the most destructive disease of rice (Oryza sativa), the blast caused by the fungus M.
oryzae is of concern worldwide. Because the pathogen staying power in the soil and crop residues
for long and indeterminate periods is essential monitoring before the pathogen's entry into new
areas, due to the commitment of nearby plantations. The seeds are considered the main ways of
spread of disease due to storage, transportation and introduction into harmless areas. The
methodology currently recommended by the Ministry of Agriculture for fungi monitoring seed is
the "Blotter test", however, the morphological characterization can often be uncertain due to the
rapid growth of fungal invasion and the similarity between structures of some pathogens.
Morphologically M. oryzae can be indistinguishable from some species, such as the causal agent of
rice blast wheat and other grasses therefore the most adequate way for safe diagnosis is the
molecular detection. In seeds percentage infestation is often low, which is nevertheless important
because the seed of transmission capacity for the seedling, however small conidia concentrations
may be insufficient for the molecular detection by techniques potentially used as PCR (Polymerase
Chain Reaction). Technically a methodology for molecular amplification isothermal LAMP (Loop-
Mediated Isothermal Amplification) simpler recently been developed, which allows to safely
identify the target, due to the projection of specific primers based on exclusive regions of the
genome of the organism. The technique has advantages as high sensitivity due to the recognition
by the initiators of six regions of the target, in addition to specificity, speed and security in the
diagnosis. It is important to consider the cost reduction compared with PCR, due to use of constant
temperature and straightforward interpretation of results using turbidity generated in positive
samples or color change if the addition of dyes, eliminating the use of devices, specialized
professionals in handling high standard laboratories. The interpretation the results with the naked
eye makes the seed control in environments such as laboratories, warehouses, cooperatives with
minimal structure, reflecting positively on the proper targeting of lots, either for planting,
consumption or disposal. Therefore, the aim of this study was to survey the main potentially
destructive pathogenic fungi found in seeds, and develop a rapid detection kit by LAMP
methodology for M. oryzae.
Descrição
Palavras-chave
Citação
TEIXEIRA, N. C. Detecção de Magnaporthe oryzae em sementes de arroz por meio da técnica LAMP (amplificação isotérmica de ácidos nucleicos). 2016. 80 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Goiás, Goiânia, 2016.