Investigação de sapovírus em amostras de swab nasofaringeano e fezes de crianças hospitalizadas de Goiânia, Goiás
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2017-10-03
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Universidade Federal de Goiás
Resumo
Sapoviruses are important agents causing acute gastroenteritis worldwide, and are most often
detected in children under five years of age. The present study aimed to evaluate the
positivity and viral load index of sapovirus in faecal samples and nasopharyngeal swab of
children, in association with the symptoms presented by these children. The study included
102 children hospitalized at the Hospital Materno Infantil, presenting symptoms of
gastroenteritis, attended between May 2014 and May 2015, with a sample of faeces and a
nasopharyngeal swab sample from each of them. Samples of faeces and nasopharyngeal
swabs from all children were submitted to extraction of the genetic material from a
commercial kit and screened for sapovirus by RT-qPCR. The viral load was determined by
constructing a standard curve from recombinant plasmid. A faecal sample and a
nasopharyngeal swab sample were obtained and 47% of the children were positive for
sapovirus in at least one of the samples collected, being 10.7% positive only in faecal samples
and 28.4% positive only in the samples of nasopharyngeal swab. Regarding viral load, a
median of 7.77 x 108 CG / mL was found for stool samples. Regarding the viral load of the
nasopharyngeal swab samples, a median 1.54 x 108 CG / mL was observed and a statistically
significant result was observed (13/14 p = 0.01), for the samples with viral load above the
median obtained in the rainy season. The median viral load of samples from positive children
in both clinical specimens was 2.33 x 108 CG / mL in the faeces and 2.67 x 108 CG / mL in
the nasopharyngeal swab. Two of these children presented a considerably higher viral load
when compared to the others. It is hoped that the data obtained may contribute to a better
understanding of the molecular epidemiology of sapoviruses, as well as to the development of
better preventive measures, in order to limit the risk of transmission of sapoviruses,
especially in a hospital environment. This is the first study to carry out the research and
detection of sapovirus in a respiratory tract sample, which could suggest a possible alternative
route of viral transmission.
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SILVA, Thairiny Neres. Investigação de sapovírus em amostras de swab nasofaringeano e fezes de crianças hospitalizadas de Goiânia, Goiás. 2017. 74 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro ) - Universidade Federal de Goiás, Goiânia, 2017.