Uso de antioxidantes em diluentes de criopreservação de sêmen bovino
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2017-12-08
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Universidade Federal de Goiás
Resumo
The objective of this study was to evaluate the addition of antioxidants in different
cryopreservation diluents on bovine semen quality after thawing and the in vitro production of
embryos. First, the effects of adding different concentrations of glutathione (1.5 and 2.5 mM)
and melatonin (0.5 and 1.0 mM) in egg yolk semen cryopreservation diluents (Bovimix®) and
soy lecithin (Andromed®) were evaluated. The results showed that the addition of the
antioxidant glutathione did not improve the maintenance of sperm characteristics of bovine
semen. Melatonin had a negative effect on the spermatic parameters evaluated. The diluent
Andromed® obtained better parameters of spermatic kinetics when compared with the diluent
Bovimix®. However, in relation to the membrane integrity analyzes of the Bovimix® diluent
showed better performance in the maintenance of these parameters. The effect of the
cryopreservation diluents and the addition of glutathione in the vitro production of bovine
embryos (PIVE) was then evaluated. The rate of cleavage, blastocyst rate and hatching were
evaluated eight and nine days after IVF, as well as the correlation between sperm
characteristics and PIVE. The diluent Andromed® provided a better cleavage rate, with no
effect of the addition of glutathione in it. However, the addition of 2.5 mM glutathione in the
Bovimix® diluent improved the cleavage rate. There was a significant correlation of high and
low magnitude between some sperm characteristics with the rate of cleavage. It was
concluded that glutathione did not improve sperm viability. Melatonin worsened the
maintenance of sperm characteristics. Andromed® diluent was more efficient in the in vitro
production of bovine embryos with no glutathione effect observed in this diluent. The
addition of 2.5 mM glutathione in the egg yolk Bovimix® diluent gave a higher cleavage rate.
In vitro evaluation of semen quality is not the best method to predict the fertilizer potential of
the same in PIVE, because only significant low to moderate magnitude correlations were
observed between some parameters of semen quality and fertilization rate.
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SILVA, N. C. Uso de antioxidantes em diluentes de criopreservação de sêmen bovino. 2017. 95 f. Tese (Doutorado em Zootecnia) - Universidade Federal de Goiás, Goiânia, 2017.