Identificação de astrovírus não clássicos na região centro-oeste
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2019-06-26
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Universidade Federal de Goiás
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The classical human astrovirus (HAstV-1 to HAstV-8) are viruses commonly associated with
gastroenteritis in children up to five years of age. Recently new astrovirus species have been
recognized and designated as novel astrovirus, Melbourne (HAstV-MLB) and Virginia (HAstV-
VA), which have also been found in association with gastroenteritis and other more severe
clinical conditions such as meningitis and encephalitis. However, there are still few studies on
the occurrence of these pathogens in Brazil, and so far without reports in the Midwest region.
Thus, for the first time, novel astrovirus was investigated, in this region, in stool samples
obtained between May 2014 and April 2015 from children (≤6 years) with or without
symptoms of acute gastroenteritis. The children were attended at the Materno Infantil Hospital
in the city of Goiânia, Goiás. For the MLB research the reverse transcription polymerase chain
reaction (RT-PCR) was used, followed by Semi-Nested PCR targeting region 3 'end of the
ORF1b of the viral genome. It was also carried out genomic sequencing and phylogenetic
analysis for molecular characterization. From 250 samples tested, one was positive (0.4%) for
novel astrovirus and characterized as HAstV-MLB1. The sample was obtained from a 12-
month-old child with diarrhea and respiratory symptoms (cough), in addition to fever. This is
the first study to detect non-classical astrovirus in faecal samples obtained in the Midwest
region. We hope that the data obtained may contribute to a better understanding of the
molecular epidemiology and aspects of the pathogenesis of these agents in the pediatric
population.
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CAETANO, Amanda C. Identificação de astrovírus não clássicos na região centro-oeste. 2019. 68 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2019.