Interaction with Pantoea agglomerans modulates growth and melanization of Sporothrix brasiliensis and Sporothrix schenckii
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Data
2019
Autores
Paes, Rodrigo Almeida
Santos, Fabio Brito dos
Oliveira, Manoel Marques Evangelista
Bailão, Alexandre Melo
Borges, Clayton Luiz
Araújo, Glauber Ribeiro de Sousa
Frases Carvajal, Susana
Soares, Célia Maria de Almeida
Oliveira, Rosely Maria Zancopé
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Resumo
Sporothrix brasiliensis and Sporothrix
schenckii stand as the most virulent agents of
sporotrichosis, a worldwide-distributed subcutaneous
mycosis. The origin of Sporothrix virulence seems to
be associated with fungal interactions with organisms
living in the same environment. To assess this
hypothesis, the growth of these two species in
association with Pantoea agglomerans, a bacterium
with a habitat similar to Sporothrix spp., was evalu ated. Growth, melanization, and gene expression of
the fungus were compared in the presence or absence
of the bacterium in the same culture medium. Both S.
brasiliensis and S. schenckii grew in contact with P.
agglomerans yielding heavily melanized conidia after
5 days of incubation at 30 C in Sabouraud agar. This
increased melanin production occurred around bacte rial colonies, suggesting that fungal melanization is
triggered by a diffusible bacterial product, which is
also supported by a similar pattern of melanin
production during Sporothrix spp. growth in contact
with heat-killed P. agglomerans. Growth of P.
agglomerans was similar in the presence or absence
of the fungus. However, the growth of S. brasiliensis
and S. schenckii was initially inhibited, but further
enhanced when these species were co-cultured with P.
agglomerans. Moreover, fungi were able to use killed
bacteria as both carbon and nitrogen sources for
growth. Representational difference analysis identi-
fied overexpressed genes related to membrane trans port when S. brasiliensis was co-cultured with the
bacteria. The down-regulation of metabolism-related
genes appears to be related to nutrient availability
during bacterial exploitation. These findings can lead
to a better knowledge on Sporothrix ecology and
virulence.
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Pantoea agglomerans, Sporothrix brasiliensis, Sporothrix schenckii, Microbial interaction
Citação
ALMEIDA-PAES, Rodrigo et al. Interaction with Pantoea agglomerans modulates growth and melanization of Sporothrix brasiliensis and Sporothrix schenckii. Mycopathologia, Berlin, v. 184, n. 3, p. 367-381, 2019. DOI: 10.1007/s11046-019-00350-x. Disponível em: https://link.springer.com/article/10.1007/s11046-019-00350-x. Acesso em: 8 ago. 2024.