Proteômica: uma ferramenta analítica

Abstract

Proteomics is an analytical technique that allows the determination of proteins in a biological sample. Proteomic analysis is one of the most used techniques to study proteins differentially expressed in a cell when infected by microorganisms. In this way, the proteomic analysis can provide important information on the pathogen-cell relationship, pathogenesis of the microorganism, as well as contribute to the identification of target molecules that may be useful both for the development of vaccines as well as for markers, diagnostics and therapeutics. This manuscript presents a summary of different methodologies with a proteomic approach which have been used for the study of differentially expressed proteins from cells infected or uninfected by microorganisms.