Enzimas de interesse comercial isoladas de bactérias endofíticas da erva-daninha Tridax procumbens (L.) (Erva-de-Touro)

Abstract

Research with endophytic microorganisms producing different metabolites, such as hydrolytic enzymes, is increasingly present, which has great applicability in industrial processes, due to its wide variety of structure and applications, high specificity, stability, low impact on the environment, possibility large-scale production in a short period of time and at low cost. The present study aimed to isolate endophytic bacteria from Tridax procumbens (L.) and to evaluate the production of hydrolytic enzymes produced by these bacteria. A total of eight endophytic bacteria, from different parts of the plant, were selected and analyzed for their ability to synthesize the enzymes amylase, β-glucosidase, cellulase, endoglucanase, esterase, exoglucanase, lipase, pectinase, protease and xylanase, using culture specific solids for the production of each enzyme. Of the eight isolated bacteria, all showed pectinolytic and proteolytic activity, 87.5% β-glucosidase and cellulolytic activity (sugar cane), 75% endoglucanase and exoglucanase activity, 62.5% xylanase activity, 50% amylolytic activity, 37.5% cellulolytic (bamboo) and sterasic activity, none showed lipase activity. In conclusion, the isolates show potential as producers of hydrolytic enzymes of industrial interest.