Uso comparativo de amastigotas de t. cruzi et. hastatus cultivados a 35°c em meios sem células na RIFI para doenças de chagas
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1984-12
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ruy
Resumo
Amastigotes obtained form 5 days
of culture em RPMl complemented with
30% of chicken serum at 26° C and Epímastigotes
grown for 5 days in LIT culture
at 26° C of Trypanosoma cruzi and
Trypanosoma "cruzi-like" of Phyllostomus
hastatus hastatus (temporany denominalcd
T. hastatus) were used on the
classic reaction of indirect imunofluorescency (R1F1) 36 serum of patients clinicaly
chagasics, serum reactors and two of
them, parasitologicaly confirmed and 10
serum of non reactor patientes and clinicaly
healthy.
The results were analised as much
ín relation to the antigenes used as to it*s
shape. It was verified that pratically,
equal results according to the reciprocity
of the reactions titles, sensibilites, and
specificity of both antigenes and shape.
Although, the nature of the imunefluorescency
was much more clear with
the antigene of the epimastigote form
while the amastigote form was less Intense
and there was no graduation between
the titles and it became negative almost
brusque.
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OLIVEIRA, Osvaldira Seabra de; BARBOSA,William; CZEREWUTA, Ana Cândido; MENDONÇA, Joana Rosa de. Uso comparativo de amastigotas de t. cruzi et. hastatus cultivados a 35°c em meios sem células na RIFI para doenças de chagas. Revista de Patologia Tropical, Goiânia, v. 13, n.3, p. 461-466, set./dez. 1984. Disponível em: < https://www.revistas.ufg.br/iptsp/article/view/21262/12477>.