Genetic variability in natural populations of zeyheria montana mart. from brazilian cerrado
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Data
2007-08
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Escola Superior de Agricultura Luiz de Queiroz
Resumo
Zeyheria montana, an endemic species of the Bignoniaceae family from the Brazilian
Cerrado’s known for its anti-cancer properties, is widely used as imuno stimulant in the popular
medicine and its therapeutic activity must be validated by scientific data. The objective of this work
was to evaluate the genetic variability of eight plant populations collected within the state of São
Paulo, Brazil, via Random Amplification of Polymorphic DNA (RAPD) used as molecular markers.
After an optimized protocol for the amplification reaction, nine selected primers generated 105
reproducible bands, indicating up to 60% polymorphism. Analysis of molecular variance (AMOVA)
revealed higher genetic variation within populations (84.03%) than among populations (15.97%). The
variation values estimated by φST (0.160) indicated moderate to high inter population structuration.
Levels of similarity inter plants with genetic and geographical distances, estimated by the unweighted
pair-group method analysis (UPGMA) clustering and non-metric multidimensional scaling (NMDS)
ordination methods and by the Mantel test (-0.2345 p = 0.118) denoted that the structure found follows
the island model, which assumes that a single population of infinite size may have initiated the existing
populations of Zeyheria montana, with no spatial position correlation. Based on the obtained data, a
germplasm bank from individuals representing the species variability was established. Furthermore the
information here reported can be of importance to develop strategies for the conservation of Z. montana.
Key words: Bignoniaceae, germplasm bank, bolsa-de-pastor, conservation
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Palavras-chave
Bignoniaceae, Bolsa-de-pastor, Germplasm bank, Conservation
Citação
BERTONI , Bianca Waléria; ASTOLFI FILHO, Spartaco; MARTINS, Ernani Ronie; DAMIÃO FILHO, Carlos Ferreira; FRANÇA, Suzelei de Castro; PEREIRA, Ana Maria Soares; TELLES, MarianaPires de Campos; DINIZ FILHO, José Alezandre F. Genetic variability in natural populations of Zeyheria montana mart. from brazilian cerrado. Scientia Agricola, Piracicaba, v. 64, n. 4, p. 409-415, Jul./Aug. 2007.