Lecitnas leguminosas no auxílio para caracterização de leishmania

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Data

1989-12

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Editor

Ruy de Souza Lino Junior

Resumo

The polysaccharide surface of Leishmania species belonging to mexican and brasiliensis groups and isolated stocks at Gaspar Viana's laboratoy were studied by the direct agglutination test, mediated by lectins. Ten lectins' samples were prepared derived from leguminous seeds from thc following species: Lens cullinaris, Ricinus communis, Artocarpus integrifolia, Persea americana, Arachis hipogaea, Mangifera indica, Piswn sativum, Glycine max, Tamarindo, Tritiwn vulgaris, Canavalia ensiformis and Phytohemaglutinin P.; having been the last two lectins acquired in the market. Different groups could be observed related to the number and variety of the lectin's saccharide bond sites on the Leishmania surface, having the strains of brasiliensis complex shown a larger number of connected sites for the lectins comparing with the mexican complex and others isolated from patients at Gaspar Viana's laboratory. (BARBOSA, W. et. ai. 1976, 1983, 1984). We confirmed through the reaction of direct immunofluorescence that the Lens culinaris and Canavalia ensiformis marked by us with fluorescent isothiocyanate (CLARK & SHEPARD) showed similar rcsults to the test of direct agglutination. Parallelly we tested the capacity of twelve lectins to agglutinate Tripanosoma cruzi. We found bonds that the surface of Trypasonoma cruzi showed more connected bonds for the lectins, relating to the Leishmania surface. Wc belive that the new lectins plants tested are very promising in the taxonomic study of Leishmania.

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Palavras-chave

Leishmanias, Caracterização de cepas, Lectinas, Reação de aglutinação direta, Leishmania characterization, Lectins, Agglutination direci tesi

Citação

NOJIMOTO, Idalina Thiomi Inumaru; BARBOSA, William; CZEREWUTA, Ana Cândida; OLIVEIRA, Raquel Lopes de. Lecitnas leguminosas no auxílio para caracterização de leishmania. Revista de Patologia Tropical, Goiânia, v. 18, n.2, p. 173-181, jul./dez. 1989. Disponível em: < https://www.revistas.ufg.br/iptsp/article/view/20222/11772>.