Screening of fecal samples from asymptomatic children, for norovirus detection, using a third generation enzyme immunoassay commercial kit
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Data
2014-05
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Editor
Ruy de Souza Lino Junior
Resumo
Norovirus is the leading cause of non-bacterial acute gastroenteritis outbreaks worldwide.
Recently, third generation Enzyme Immunoassay (EIA) commercial kits have been developed,
and controversial results have been obtained by different studies regarding the sensitivity and
specificity of these assays. Therefore, the aim of this study was to test 60 fecal samples, previously
tested as positive by RT-PCR for caliciviruses (40 norovirus-positive and 20 sapovirus-positive
samples), for qualitative determination of genogroup I and II noroviruses by a commercial EIA
kit (RIDASCREEN® Norovirus (C1401) 3rd Generation, R-Biopharm, Darmstadt, Germany). The
samples were obtained from 30 children aged less than five years, mostly asymptomatic, who attend
a day-care center in Goiânia, Goiás, Brazil. The results conferred a positivity rate for NoV of 35%
and a specificity rate of 100% for the EIA, when compared to the RT-PCR. The test also failed to
detect samples that were positive for GI.1 and GI.4 norovirus. The presumably lower viral load of
asymptomatic children might be related to the poor sensitivity. Our results reinforce the notion that
screening of samples by molecular assays, especially of samples that might have a low number of
viral particles such as those obtained from asymptomatic patients, should not be replaced by the use
of EIA kits.
Descrição
Palavras-chave
Asymptomatic children, Enzyme immunoassay, Norovirus, Sensitivity, Specificity, Crianças assintomáticas, Ensaio imunoenzimático, Sensibilidade, Especificidade
Citação
SANTOS, Hugo César Pereira; TURONES, Larissa Córdova; FIACCADORI, Fabíola Souza; CARDOSO, Divina das Dôres de Paula; SOUZA, Menira Borges de Lima Dias e. Screening of fecal samples from asymptomatic children, for norovirus detection, using a third generation enzyme immunoassay commercial kit. Revista de Patologia Tropical, Goiânia, v. 43, n. 2, p. 143-149 abr./jun. 2014. Disponível em: <https://www.revistas.ufg.br/iptsp/article/view/31111/16786>.