Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/11993
Tipo do documento: Artigo
Título: Genetic diversity in natural populations of Jacaranda decurrens Cham. determined using RAPD and AFLP markers
Autor: Bertoni, Bianca Waleria
Telles, Mariana Pires de Campos
Gaiosso, Milena Gaion
Torres, Simone Cristina Zampollo
Pereira, José Odair
Lourenço, Miriam Verginia
França, Suzelei de Castro
Pereira, Ana Maria Soares
Abstract: Jacaranda decurrens (Bignoniaceae) is an endemic species of the Cerrado with validated antitumoral activity. The genetic diversity of six populations of J. decurrens located in the State of São Paulo was determined in this study by using molecular markers for randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). Following optimization of the amplification reaction, 10 selected primers generated 78 reproducible RAPD fragments that were mostly (69.2%) polymorphic. Two hundred and five reproducible AFLP fragments were generated by using four selected primer combinations; 46.3% of these fragments were polymorphic, indicating a considerable level of genetic diversity. Analysis of molecular variance (AMOVA) using these two groups of markers indicated that variability was strongly structured amongst populations. The unweighted pair group method with arithmatic mean (UPGMA) and Pearson’s correlation coefficient (RAPD -0.16, p = 0.2082; AFLP 0.37, p = 0.1006) between genetic matrices and geographic distances suggested that the population structure followed an island model in which a single population of infinite size gave rise to the current populations of J. decurrens, independently of their spatial position. The results of this study indicate that RAPD and AFLP markers were similarly efficient in measuring the genetic variability amongst natural populations of J. decurrens. These data may be useful for developing strategies for the preservation of this medicinal species in the Cerrado.
Palavras-chave: Bignoniaceae
Carobinha
Cerrado
Germplasm bank
Preservation
País: Brasil
Instituição: Sociedade Brasileira de Genética
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: BERTONI, Bianca W.; TELLES, Mariana P. de C.; MALOSSO, Milena G.; TORRES, Simone C. Z.; PEREIRA, José O.; LOURENCO, Mirian V.; FRANCA, Suzilei de C.; PEREIRA, Ana M. S. Genetic diversity in natural populations of Jacaranda decurrens Cham. determined using RAPD and AFLP markers. Genetics and Molecular Biology, Ribeirão Preto, v. 33, n. 3, p. 532-538, Sept. 2010.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S1415-47572010005000068
Identificador do documento: 10.1590/S1415-47572010005000068
URI: http://repositorio.bc.ufg.br/handle/ri/11993
Data de publicação: Set-2010
Aparece nas coleções:ICB - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Bianca Waleria Bertoni - 2013.pdf164,74 kBAdobe PDFThumbnail
Baixar/Abrir


Este item está licenciada sob uma Licença Creative Commons Creative Commons