Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae)
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Data
2013-02
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Fundação de Pesquisas Científicas de Ribeirão Preto
Resumo
Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles per locus (primer) ranged from 3 to 11 and expected heterozygosities varied from 0.309 to 0.884. The probability of locus identity was ~1.88 x 10-4 and the probability of paternity exclusion was ~0.9367. The 5 microsatellite primer pairs may be suitable for population genetic studies such as parentage and fine-scale genetic analyses of this species.
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Palavras-chave
Cagaita, Cerrado, Genetic diversity, Shotgun library, Microsatellite
Citação
TELLES, M. P. C.; SILVA, J. B.; RESENDE, L. V.; VIANELLO, R. P.; CHAVES, L. J.; SOARES, T. N.; COLLEVATTI, R. G. Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae). Genetics and Molecular Research, Ribeirão Preto, v. 12, n. 3, p. 3124-3127, Feb. 2013.