Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/12043
Tipo do documento: Artigo
Título: Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae)
Autor: Telles, Mariana Pires de Campos
Peixoto, Franciele Parreira
Lima, Jacqueline de Souza
Resende, Lucileide Vilela
Vianello, Rosana Pereira
Walter, Maria Emília Machado Telles
Collevati, Rosane Garcia
Abstract: We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in “cerrado rupestre” from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer design. Leaves from 96 individuals collected in the three known local populations were genotyped using the 12 primers designed to analyze the polymorphisms at each locus. The number of alleles per locus ranged from one to six; two loci were monomorphic. Among the polymorphic loci, expected heterozygosities ranged from 0.161 to 0.714. Combined paternity exclusion probability was 0.957 and combined genetic identity (0.051) was high for studies on parentage. Tibouchina papyrus is a rare and endemic tree species of outcrop quartzite and sandstone soils, with highly isolated populations, which may have lead to the low degree of polymorphism that we detected. Also, motifs of most loci are larger than dinucleotide, which typically display lower levels of polymorphism.
Palavras-chave: Cerrado
Pau-papel
Shotgun library
SSR
STR
País: Brasil
Instituição: Fundação de Pesquisas Científicas de Ribeirão Preto
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: TELLES, M. P. C.; PEIXOTO, F. P.; LIMA, J. S.; RESENDE, L. V.; VIANELLO, R. P.; WALTER, M. E. M. T.; COLLEVATTI, R. G. Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae). Genetics and Molecular Research, Ribeirão Preto, v. 10, n. 1, p. 321-325, Feb. 2011.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.4238/vol10-1gmr1026
Identificador do documento: 10.4238/vol10-1gmr1026
URI: http://repositorio.bc.ufg.br/handle/ri/12043
Data de publicação: Fev-2011
Aparece nas coleções:ICB - Artigos publicados em periódicos

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