Caracterização bioquímica do produto de excreção/ secreção de larvas de cochliomyia hominivorax (diptera: calliphoridae)
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Data
2016-09
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Editor
José Henrique Stringhini
Resumo
The species Cochliomyia hominivorax, also known as screwworm fly, is an obligate parasite of warmblooded
animals and its geographic range extends thoughout South America, except Chile. This fly
causes significant economic losses and has great importance in Brazil. Few studies have focused on
the excretion and secretion products of this species, and this research aimed to study the enzymes
present in the secretion and excretion (E/S) products of the three larval instars of C. hominivorax.
The E/S profile of proteins was obtained by polyacrylamide gel electrophoresis and proteolytic
activity was analyzed using gelatin, azocasein and Na-benzoyl-arginine-nitroanilide as substrates.
In E/S products of the three instars, proteins were detected with an apparent molecular weight ranging between 116 and 20 kDa. In the azocasein assay, at different pH ranges, the major proteolytic
activity occurred at pH 7.5 for all larval instars. Assays were performed using the same substrates
in which the samples were treated with the inhibitors Benzamidine, Pepstatin A, 4-(2-Aminoethyl)
benzenesulfonyl fluoride hydrochloride (AEBSF), N-α-tosyl-L-lysine chloromethyl ketone (TLCK),
N-α- tosyl-L-phenylalanine chloromethyl ketone (TPCK), Ethylenediamine tetraacetic acid (EDTA),
and Leupeptin-trans-Epoxysuccinyl-leucylamido(4-guanidino) butane (E-64). Proteinases present in
the E/S product of first larvae instar are mostly serine trypsin and chymotrypsin proteases, whereas
for second and third instars serine proteases and aspartyl proteases were predominantly observed.
Biochemical characterization of E/S products of all larval stages of C. hominivorax helps to improve
the understanding of the physiology and the interaction of this parasite with host tissues.
Descrição
Palavras-chave
Enzima, Miíase, Mosca, Parasitas, Enzyme, Fly, Myiasis, Parasites
Citação
TEIXEIRA, Denise Gonçalves; BORGES, Lígia Miranda Ferreira; MASTRÂNGELO, Thiago de Araújo; MONTEIRO, Valdirene Neves. Caracterização bioquímica do produto de excreção/ secreção de larvas de cochliomyia hominivorax (diptera: calliphoridae). Ciência Animal Brasileira, Goiânia, v. 17, n. 4, p. 581-592, jul./set. 2016. Disponível em: <https://www.revistas.ufg.br/vet/article/view/37176/21919>.