Comparação de métodos de descontaminação usados na fase inicial do estabelecimento em cultura in vitro de banana

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Data

1999

Título da Revista

ISSN da Revista

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Editor

Antônio Henrique Garcia

Resumo

Four decontamination methods for in vitro culture of ba­nana (Musa AAB cv. Maçã) explants were compared. Commercial crop rhizomes were used as the explant source. Different sizes of explants obtained from ax:illary buds were submersed in different concentrations ofNaOCI for each different period oftime. A:fter being reduced to 8mm X 5mm x 5mm, explants were transfered to modified MS media, which were kept in the dark for 10 days, followed by growth chamber at28ºC ± 2ºC with l 6h \ight. Best results were obtained with 40mm x 20mm x 20mm or 40mm x 15mm x 15mm explants, immersed once or twice in 3,5% NaOCl. Higher contamination leveis were observed 15 days after the beginning of the experiment; at 1 % NaOCI, however, 96,67% contamination was observed after 3 days. Bacterial incidence was higher than fungai.

Descrição

Palavras-chave

Banana cv. maçã, Cultura de tecidos, Es­terilização superficial, Musa AAB, Tissue culture, Superficial sterilization

Citação

CARNEIRO, Iraídes Fernandes; ZICA, Lincoln Fonseca; CHAVES, Lázaro José. Comparação de métodos de descontaminação usados na fase inicial do estabelecimento em cultura in vitro de banana. Pesquisa Agropecuária Tropical, Goiânia, v. 29, n. 2, p. 89-94, jul./dez. 1999. Disponível em: <https://www.revistas.ufg.br/pat/article/view/2856/2906>.