Comparação de métodos de descontaminação usados na fase inicial do estabelecimento em cultura in vitro de banana
Carregando...
Data
1999
Título da Revista
ISSN da Revista
Título de Volume
Editor
Antônio Henrique Garcia
Resumo
Four decontamination methods for in vitro culture of banana (Musa AAB cv. Maçã) explants were compared. Commercial crop rhizomes were used as the explant source. Different sizes of explants obtained from ax:illary buds were submersed in different concentrations ofNaOCI for each different period oftime. A:fter being reduced to 8mm X 5mm x 5mm, explants were transfered to modified MS media, which were kept in the dark for 10 days, followed by growth chamber at28ºC ± 2ºC with l 6h \ight. Best results were obtained with 40mm x 20mm x 20mm or 40mm x 15mm x 15mm explants, immersed once or twice in 3,5% NaOCl. Higher contamination leveis were observed 15 days after the beginning of the experiment; at 1 % NaOCI, however, 96,67% contamination was observed after 3 days. Bacterial incidence was higher than fungai.
Descrição
Palavras-chave
Banana cv. maçã, Cultura de tecidos, Esterilização superficial, Musa AAB, Tissue culture, Superficial sterilization
Citação
CARNEIRO, Iraídes Fernandes; ZICA, Lincoln Fonseca; CHAVES, Lázaro José. Comparação de métodos de descontaminação usados na fase inicial do estabelecimento em cultura in vitro de banana. Pesquisa Agropecuária Tropical, Goiânia, v. 29, n. 2, p. 89-94, jul./dez. 1999. Disponível em: <https://www.revistas.ufg.br/pat/article/view/2856/2906>.