Obtenção de células mononucleares da medula óssea pela punção do tubérculo umeral de coelhos
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Data
2010
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Resumo
Bone marrow derived-mesenchymal stem cells have been isolated and used for osteochondral defects regeneration and
repair. The aim of this study was evaluate the efficiency of bone marrow harvest technique using twelve adult male
New Zealand rabbits, with weight between 3.0 kg to 4.5 kg. After shoulder joint flexion, a heparinized Rosenthal
biopsy needle was inserted through the humeral tubercle and further aspiration of 2.0 mL bone marrow. In the isolation
with Ficoll-paque gradient density, was achieved a mean yield of 6.25 x 106 mononuclear cells per milliliter. The bone
marrow harvest technique by puncture of the humerus tubercle was easily accomplished and effective, due to high yield
of isolated mononuclear cells of samples.
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Células mesenquimais, Colheita, Medula óssea, Rendimento, Úmero, Bone marrow, Harvest, Humerus, Mesenchymal cells, Yield
Citação
EURIDES, D.; OLIVEIRA, B. J. N. A.; SOUZA, L. A.; SILVA, L. A. F.; DALECK, C. R.; FREITAS, P. M. C. Obtenção de células mononucleares da medula óssea pela punção do tubérculo umeral de coelhos. ARS Veterinária, Jaboticabal, v. 26, n. 2, p. 71-76, 2010.