Isolamento, identificação e avaliação das atividades enzimática e antibacteriana de micro-organismos endofíticos de Hyptis suaveolens (L.) Poit
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Data
2015
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Resumo
Endophytic are microorganisms that spent part or all the life cycle inside vegetal
tissues. Under stress conditions, as hydric deficit, phytopathogen invasion or contact
with toxic substances, they generate diverse biomolecules that aid in the vegetal
protection. The actual study aimed to isolate and evaluate the enzymatic and
antimicrobial potential of endophytic microorganisms from Hyptis suaveolens (L.)
Poit., a vegetal species broadly applied in the Brazilian phytotherapy. A total of 39
microbial isolates were selected from different parts of the plants. After the
identification, it was found the most part of the isolated microorganisms were bacteria
belonging to the genera Pseudomonas sp. (23,1%), Bacillus sp. (23,0%),
Streptomyces sp. (20.5%), Alcaligenes sp. (17,9%), Micrococcus sp. (5,1%),
Comamonas sp. (2,6%), Acinetobacter sp. (2,6%) and Staphylococcus sp. (2,6%),
and a yeast from the genus Rhodotorula sp. (2.6%). At least one type of a
exoenzyme action (amylase, lipase, esterase, pectinase or protease) was observed
for 25 isolates (64,1%). Concerning the in vitro screening of the antimicrobial activity,
it was observed 15 isolates (38,5%) exhibited capacity to inhibit the growth of some
of the 16 target microorganisms, especially the strains Pseudomonas aeruginosa MC
7B and Pseudomonas fluorescens MC 15, which presented the highest level of
antibiotic action against 15 (93,8%) and 9 (56,2%) of the indicator bacterial strains,
respectively. In conclusion, the study highlights the endophytic community from this
medicinal plant exhibits a profitable potencial for the generation of enzymes of
industrial interest and antimicrobials.
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Palavras-chave
Micro-organismos endofíticos, Hyptis suaveolens (L.) Poit, Enzimas, Atividade antibacteriana, Bioprospecção, Endophytic microorganisms, Enzymes, Antibacterial activity, Bioprospecting
Citação
BARBOSA, Edweis Cândida; CARRIM, Aysha Jussara Ivonilde; OLIVEIRA, Bruno Francesco Rodrigues de; RIBEIRO, Igor Daniel Alves; VIEIRA, José Daniel Gonçalves. Isolamento, identificação e avaliação das atividades enzimática e antibacteriana de micro-organismos endofíticos de Hyptis suaveolens (L.) Poit. Enciclopédia Biosfera, Goiânia, v. 11, n. 22, p. 3036-3055, 2015.