Precision micro-spray tower for application of entomopathogens
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Data
2013
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Resumo
An inexpensive, portable and easy handling spraying device was developed for
experimental application of water suspensions of entomopathogens and insecticides. The micro-sprayer
was calibrated using different settings of pressure and time to apply a wide range of doses on a plain
surface. Filter paper cards sprayed with dye solutions were used to examine the deposition uniformity
(coverage). The relationship between conidial suspensions of fungal entomopathogens and deposition
rate of conidia was also investigated. As a result, the combination of 10 PSI and 3 s provided the most
uniform coverage at a volume application rate of 2.15 μL cm -2 (= 215 L ha -1 ). Conidial deposition
rate was positively linear to fungal concentrations and regression lines were not significantly different
among the fungal species tested. Hence, a single regression line was determined, which gave an estimate
of 150 conidia mm -2 (= 1.5×10 12 conidia ha -1 ) based on a concentration of 1×10 7 conidial mL -1 . Once
calibrated, this spray device can be used in a variety of bioassays testing efficacy of entomopathogens
and chemical pesticides in small confined experimental arena.
Descrição
Palavras-chave
Controle biológico, Bioensaios, Sistema de pulverização, Fungos entomopatogênicos, Biological control, Entomopathogenic fungi, Spray system, Bioassays
Citação
MASCARIN, Gabriel Moura; QUINTELA, Eliane Dias; SILVA, Eli Gonçalves da; ARTHURS, Steven Paul. Precision micro-spray tower for application of entomopathogens. BioAssay, Piracicaba, v. 8, n. 1, p. 1-8, 2013.