Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/14174
Tipo do documento: Artigo
Título: Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
Autor: Britto, Constança
Silveira, Celeste
Cardoso, Maria Angelica
Marques, Patricia
Ostermayer, Alejandro Luquetti
Macêdo, Vanize
Fernandes, Octavio
Resumo: Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and 48 in the chronic phase. Fifteen chronic assymptomatic patients received a placebo. Treatment in the acute phase led to PCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%. In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which 36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals had their antibodies titers decreased to such a level that the final results were considered as doubtful and 16 of them presented negative PCR. The molecular method represents a clear advantage over conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent chemotherapy.
Palavras-chave: Trypanosoma cruzi
Kinetoplast DNA
Chagas disease
PCR
Treatment
País: Brasil
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: BRITTO, Constança; SILVEIRA, Celeste; CARDOSO, Maria Angelica; MARQUES, Patricia; LUQUETTI, Alejandro; MACÊDO, Vanize; FERNANDES, Octavio. Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n.6, p. 823-826, Aug. 2001.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S0074-02762001000600014
Identificador do documento: 10.1590/S0074-02762001000600014
URI: http://repositorio.bc.ufg.br/handle/ri/14174
Data de publicação: Ago-2001
Aparece nas coleções:FM - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Constança Britto - 2001.pdf40,17 kBAdobe PDFThumbnail
Baixar/Abrir


Este item está licenciada sob uma Licença Creative Commons Creative Commons