Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/14175
Tipo do documento: Artigo
Título: Serodiagnosis of Trypanosoma cruzi using the new particle gel immunoassay - ID-PaGIA Chagas
Autor: Rabello, Ana
Ostermayer, Alejandro Luquetti
Moreira, Eliana F.
Gadelha, Maria de Fátima
Santos, José Alisson dos
Melo, Laércio de
Schwind, Peter
Resumo: The ID-Chagas test is a particle gel immunoassay (PaGIA). Red coloured particles are sensitised with three different synthetic peptides representing antigen sequences of Trypanosoma cruzi: Ag2, TcD and TcE. When these particles are mixed with serum containing specific antibodies, they agglutinate. The reaction mixture is centrifuged through a gel filtration matrix allowing free agglutinated particles to remain trapped on the top or distributed within the gel. The result can be read visually. In order to investigate the ability of the ID-PaGIA to discriminate negative and positive sera, 111 negative and 119 positive, collected in four different Brazilian institutions, were tested by each of the participants. All sera were previously classified as positive or negative according to results obtained with three conventional tests (indirect immunofluorescence, indirect hemaglutination, and enzime linked immunosorbent assay). Sensitivity rates of ID-PaGIA varied from 95.7% to 97.4% with mean sensitivity of 96.8% and specificity rates varied from 93.8 to 98.8% with mean specificity of 94.6%. The overall Kappa test was 0.94. The assay presents as advantages the simplicity of operation and the reaction time of 20 min. In this study, ID-PaGIA showed to be highly sensitive and specific.
Palavras-chave: Chagas’ disease
Immunodiagnosis
Serological tests
Particle gel immunoassay
País: Brasil
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: RABELLO, Ana; LUQUETTI, Alejandro O.; MOREIRA, Eliana F.; GADELHA, Maria de Fátima; SANTOS, José Alisson dos; MELO, Laércio de; SCHWIND, Peter. Serodiagnosis of Trypanosoma cruzi using the new particle gel immunoassay - ID-PaGIA Chagas. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 94, n.1, p. 77-82, Jan. 1999.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S0074-02761999000100016
Identificador do documento: 10.1590/S0074-02761999000100016
URI: http://repositorio.bc.ufg.br/handle/ri/14175
Data de publicação: Jan-1999
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