Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/14498
Tipo do documento: Artigo
Título: Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp.
Autor: Sena, Ana Paula Alves de
Chaibub, Amanda Abdallah
Cortes, Márcio Vinícius de Carvalho Barros
Silva, Gisele Barata da
Lobo, Valacia Lemes da Silva
Prabhu, Anne Sitarama
Filippi, Marta Cristina Corsi de
Araújo, Leila Garcês de
Resumo: Epicoccum sp. showed in vitro antagonism to the rice pathogens Magnaporthe oryzae, Rhizoctonia solani, Sarocladium oryzae, Monographella albescens and Cochliobolus miyabeanus in dual culture. The colony growth of the rice blast fungus, M. oryzae, was reduced by 42.50%. The lethal doses (LD 50 ) determined based on probit-log dosage response curves at 3 and 6 hours after germination were 393.0 and 326.6 ppm, respectively. The crude extract (600 ppm) reduced appressorial formation by 95.68%. A greenhouse experiment comparing the relative efficiency of conidial suspension and crude extract of Epicoccum sp. in the suppression of leaf blast showed no statistical difference between both application methods. However, the crude extract of Epicoccum sp. (4000 ppm) 48 hours before the application of M. oryzae induced resistance and suppressed leaf blast by 97.6%. Scanning electron microscopy of rice leaves inoculated with crude extract of Epicoccum sp. and challenged with M. oryzae showed appressorial deformation on penetration phase. Peroxidase and β-1,3-glucanase activities increased in plants sprayed with crude extract of Epicoccum sp., 24 hours after the application of the challenger. PAL as well as chitinase activities increased 72 hours after challenge inoculation. Epicoccum sp. was shown to be a potential antagonist and inducer of resistance against M. oryzae.
Palavras-chave: Epicoccum sp.
PR proteins
Magnaporthe oryzae
Oryza sativa
Antagonism
ISR
País: Brasil
Unidade acadêmica: Escola de Agronomia - EA (RG)
Citação: SENA, Ana P. A.; CHAIBUB, Amanda A.; CÔRTES, Márcio V. C. B.; SILVA, Gisele B.; SILVA-LOBO, Valácia L.; PRABHU, Anne S.; FILIPPI, Marta C. C.; Araújo, Leila G. Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp. Tropical Plant Pathology, Brasília, v. 38, n. 5, p. 387-397, 2013.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S1982-56762013005000028
Identificador do documento: 10.1590/S1982-56762013005000028
URI: http://repositorio.bc.ufg.br/handle/ri/14498
Data de publicação: 2013
Aparece nas coleções:EA - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Ana Paula Alves de Sena - 2013.pdf18,92 MBAdobe PDFThumbnail
Baixar/Abrir


Este item está licenciada sob uma Licença Creative Commons Creative Commons