Análise do polimorfismo no gene CYP2C29 em camundongos Swiss e sua influência no tratamento do câncer
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Data
2012-09
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Resumo
Present study performed an allelic identification of CYP2C9 gene on 19 SWISS
mice trying to determine a genetic profile of mice group that can be used to posterior
drug metabolism studies. Blood samples were collected and transferred to microtubes
CONCLUSÃOwith heparin. DNA extractions were carried out by saline solution and chlorophorm.
DNA samples were submitted to PCR using specific primers to genome region of murine
CYP2C29 gene (homologous of human CYP2C9 gene). Wild allele of CYP2C29*1 was
found on 21% of samples while mutated alleles were found on 79%. Results revealed
that mutant alleles are more frequent when compared to wild type. Results of present
work are important to future studies involving drug metabolism using mice lineages.
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Farmacogenômica, Metabolismo, Farmacogenética, Câncer, Citocromo, Camundongo, Pharmacogenomics, Cytochrome, Pharmacogenetics, Cancer, Metabolism, Mouse
Citação
PEREIRA, Lucas Carlos G.; VILANOVA-COSTA, Cesar Augusto S.; ARAÚJO, Garcês de; SILVEIRA-LACERDA, Elisângela de Paula. Análise do polimorfismo no gene CYP2C29. Estudos, Goiânia, v. 39, n. 3, p. 321-330, jul./set. 2012.