Use este identificador para citar ou linkar para este item:
Tipo do documento: Artigo
Título: Validation of a rapid immunochromatographic assay for diagnosis of Trypanosoma cruzi infection among latin-american migrants in Geneva, Switzerland
Autor: Chappuis, François
Mauris, Anne
Holst, Marylise
Albajar-Vinas, Pedro
Jannin, Jean
Ostermayer, Alejandro Luquetti
Jackson, Yves
Abstract: Chagas’ disease is a global public health problem due to the recent exchange of population between Latin America and other regions, including Europe. The recent development of rapid diagnostic tests (RDTs) for Trypanosoma cruzi infection may improve patient access to diagnosis and care worldwide. We evaluated the diagnostic accuracy of the Chagas Stat-Pak RDT in a cohort of undocumented Latin-American migrants living in Geneva, Switzerland. Study participants were enrolled in a primary health care center. The Chagas Stat-Pak test was performed independently on blood and serum samples. A combination of two commercialized enzymelinked immunosorbent assay (ELISA)-based serological tests was used for comparison (reference standard). A total of 999 adults (median age, 36 years) were included in the study; the majority were women (83%) and originally from Bolivia (47%) or Brazil (25%). A total of 125 participants (12.5%) were diagnosed with T. cruzi infection; with the exception of three individuals, all individuals diagnosed with T. cruzi were originally from Bolivia. The sensitivity and specificity of the Chagas Stat-Pak test on blood samples were 95.2% (95% confidence interval [95% CI], 89.2% to 97.9%) and 99.9% (95% CI, 99.3% to 100%), respectively. When the test was performed on serum samples, the sensitivity was 96% (95% CI, 91% to 98.3%), and the specificity was 99.8% (95% CI, 99.2% to 99.9%). The concordance of test results for blood and serum samples was 99.7%. Both negative and positive predictive values were above 98%. The Chagas Stat-Pak is an accurate diagnostic test for T. cruzi infection among Latin-American migrants living in Europe. The mild deficit in sensitivity should be interpreted in light of its ease of use and capacity to provide immediate results, which allow more people at risk to have access to diagnosis and care both in countries where Chagas’ disease is endemic and in countries where this disease is not endemic.
Palavras-chave: Immunochromatographic
Trypanosoma cruzi
Chagas disease
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: CHAPPUIS, François; MAURIS, Anne; HOLST, Marylise; ALBAJAR-VINAS, Pedro; JANNIN, Jean; LUQUETTI, Alejandro O.; JACKSON, Yves. Validation of a rapid immunochromatographic assay for diagnosis of Trypanosoma cruzi infection among latin-american migrants in Geneva, Switzerland. Journal of Clinical Microbiology, Washington, v. 48, n. 8, p. 2948-2952, Aug. 2010.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1128/JCM.00774-10
Identificador do documento: 10.1128/JCM.00774-10
Data de publicação: Ago-2010
Aparece nas coleções:FM - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - François Chappuis - 2010.pdf180,78 kBAdobe PDFThumbnail

Este item está licenciada sob uma Licença Creative Commons Creative Commons