Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/14655
Tipo do documento: Artigo
Título: Validation of a rapid and reliable test for diagnosis of Chagas´disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America
Autor: Ponce, Carlos
Ponce, Elisa
Vinelli, Elizabeth
Montoya, Alberto
Aguilar, Vilma de
Gonzalez, Antonio
Zingales, Bianca
Rangel-Aldão, Rafael
Levin, Mariano Jorge
Esfandiari, Javan
Umezawa, Eufrosina Setsu
Ostermayer, Alejandro Luquetti
Silveira, José Franco da
Abstract: In this study we compared the performance of the Chagas Stat-Pak rapid immunochromatographic test with a standard enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of Chagas’ disease in Central America. Out of 3,400 blood donor samples, 156 (4.6%) were positive in both assays. Three sera out of 2,084 samples from reference laboratories were negative with the rapid test but positive with the ELISA (99.8% agreement). Agreement of 100% between the two tests was observed with 339 additional sera from patients with cardiopathies and 175 sera from potential blood donors in emergency surgical cases occurring on weekends or at night. In conclusion, Chagas Stat-Pak showed 99.6% and 99.9% sensitivity and specificity, respectively, when assayed with 5,998 serum samples. It is a sensitive and specific alternative to the ELISA, as required in medical emergencies and blood screenings in Central America.
Palavras-chave: Test for diagnosis
Trypanosoma cruzi
Chagas disease
Antibodies in blood
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: PONCE, Carlos; PONCE, Elisa; VINELLI, Elizabeth; MONTOYA, Alberto; AGUILAR, Vilma de; GONZALEZ, Antônio; ZINGALES, Bianca; RANGEL-ALDÃO, Rafael; LEVIN, Mariano J.; ESFANDIARI, Javan; UMEZAWA, Eufrosina S.; LUQUETTI, Alejandro O.; SILVEIRA, José Franco da. Validation of a rapid and reliable test for diagnosis of Chagas´disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America. Journal of Clinical Microbiology, Washington, v. 43, n. 10, p. 5065-5068, Oct. 2005.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1128/JCM.43.10.5065–5068.2005
Identificador do documento: 10.1128/JCM.43.10.5065–5068.2005
URI: http://repositorio.bc.ufg.br/handle/ri/14655
Data de publicação: Out-2005
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