Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/14719
Tipo do documento: Artigo
Título: Radiolabed total parasite DNA probe specifically detects Trypanosoma cruzi in mammalian blood
Autor: Ashall, Frank
Yip-Chuck, Deborah
Ostermayer, Alejandro Luquetti
Miles, Michael
Abstract: A DNA-DNA hybridization procedure is described in which radiolabeled total parasite DNA is used to detect Trypanosoma cruzi spotted directly onto nylon membrane. Under conditions that favor hybridization of repetitive DNA sequences, the radiolabeled total DNA was able to detect as few as five parasites spotted onto nylon membrane. Trypanosomes were detectable in blood from mice with T. cruzi parasitemias spotted directly onto membranes. Ali 10 strains of T. cruzi examined, which came from different areas of the United States and South America, were readily detected with a total DNA probe from any 1 strain of the parasite. No signals were detected with up to 20,000 Trypanosoma rangeli, T. brucei, Crithidia fasciculata, or Leishmania parasites or with normal mouse blood or mammalian DNA. The hybridization method is sensitive, specific, rapid, and inexpensive and is potentially applicable to the detection of other parasites.
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: ASHALL, Frank; YIP-CHUCK, Deborah A. M.; LUQUETTI, Alejandro O.; MILES, Michael A. Radiolabed total parasite DNA probe specifically detects Trypanosoma cruzi in mammalian blood. Journal of Clinical Microbiology, Washington, v. 26, n. 3, p. 576-578, Mar. 1988.
Tipo de acesso: Acesso Aberto
URI: http://repositorio.bc.ufg.br/handle/ri/14719
Data de publicação: Mar-1988
Aparece nas coleções:FM - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Frank Ashall - 1988.pdf571,55 kBAdobe PDFThumbnail
Baixar/Abrir


Este item está licenciada sob uma Licença Creative Commons Creative Commons