Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/14830
Tipo do documento: Artigo
Título: Selection and optimization of extracellular lipase production using agro-industrial waste
Autor: Almeida, Pabline Rafaella Mello Bueno de
Oliveira, Tatianne Ferreira de
Caliari, Márcio
Castiglioni, Gabriel Luis
Soares Júnior, Manoel Soares
Abstract: The aim of this study was to isolate and select lipase-producing microorganisms originated from different substrates, as well as to optimize the production of microbial lipase by submerged fermentation under different nutrient conditions. Of the 40 microorganisms isolated, 39 showed a halo around the colonies and 4 were selected (Burkholderia, Bacillus sp., Penicillium lanosum and Corynebac glutamyl), where strains showing lipolytic halo Radius (R) / colony radius (r) ratio greater than 2.0 were selected. The results of submerged fermentation expressed as enzymatic activity revealed that the genera of microorganisms significantly influenced the enzymatic reaction, and lipase obtained from Burkholderia cepacia was the most promising, with activity of 0.0058 U.mL -1 . It was also observed in the optimization step of lipase production that the sodium nitrate content (NaNO 3 ) had a positive effect on enzyme production, and its increase was indicative of higher enzymatic activity. The addition of sources of organic nitrogen (corn steep liquor, p = 0.2398), carbon (soybean oil, p = 0.3379), magnesium MgSO 4 .7H 2 O (p = 0.4189) and potassium KH 2 PO 4 (p = 0.8562) had no significant effects on the lipase production and could result in decreased production of extracellular lipases.
Palavras-chave: Burkholderia cepacia
Residue
Hydrolytic enzymes
Submerged fermentation
País: Outros
Unidade acadêmica: Escola de Agronomia - EA (RG)
Citação: BUENO, Pabline Rafaella Mello; OLIVEIRA, Tatianne Ferreira de; CALIARI, Márcio; CASTIGLIONI, Gabriel Luis; SOARES JÚNIOR, Márcio Soares. Selection and optimization of extracellular lipase production using agro-industrial waste. African Journal of Biotechnology, Lagos, v. 13, n. 4, p. 566-573, Jan. 2014.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.5897/AJB2013.13453
Identificador do documento: 10.5897/AJB2013.13453
URI: http://repositorio.bc.ufg.br/handle/ri/14830
Data de publicação: Jan-2014
Aparece nas coleções:EA - Artigos publicados em periódicos

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