Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/14935
Tipo do documento: Artigo
Título: Paracoccidioides spp .ferrous and ferric iron assimilation pathways
Autor: Bailão, Elisa Flávia Luiz Cardoso
Lima, Patrícia de Sousa
Bailão, Mirelle Garcia Silva
Bailão, Alexandre Melo
Fernandes, Gabriel da Rocha
Kosman, Daniel Jacob
Soares, Célia Maria de Almeida
Abstract: Iron is an essential micronutrient for almost all organisms, including fungi. Usually, fungi can uptake iron through receptor-mediated internalization of a siderophore or heme, and/or reductive iron assimilation (RIA). Traditionally, the RIA pathway consists of ferric reductases (Fres), ferroxidase (Fet3) and a high-affinity iron permease (Ftr1). Paracoccidioides spp. genomes do not present an Ftr1 homolog. However, this fungus expresses zinc regulated transporter homologs (Zrts), members of the ZIP family of membrane transporters that are able in some organisms to transport zinc and iron. A 2,3,5-triphenyltetrazolium chloride (TTC)-overlay assay indicates that both Pb01 and Pb18 express a ferric reductase activity; however, 59Fe uptake assays indicate that only in Pb18 is this activity coupled to a reductase-dependent iron uptake pathway. In addition, Zrts are up-regulated in iron deprivation, as indicated by RNAseq and qRT-PCR using Pb01 transcripts. RNAseq strategy also demonstrated that transcripts related to siderophore uptake and biosynthesis are up-regulated in iron-deprived condition. The data suggest that the fungus could use both a non-classical RIA, comprising ferric reductases and Fe/Zn permeases (Zrts), and siderophore uptake pathways under iron-limited conditions. The study of iron metabolism reveals novel surface molecules that could function as accessible targets for drugs to block iron uptake and, consequently, inhibit pathogen's proliferation.
Palavras-chave: Paracoccidioidomycosis
Iron reduction
Iron uptake
Ferric reductase
Zinc-regulated transporter
Multicopper oxidases
País: Suica
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: BAILÃO, Elisa Fávia L. C. et. al. Paracoccidioides spp .ferrous and ferric iron assimilation pathways. Frontiers in Microbiology, Lausanne, v. 6, n. 821, p. 1-12, 2015.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.3389/fmicb.2015.00821
Identificador do documento: 10.3389/fmicb.2015.00821
URI: http://repositorio.bc.ufg.br/handle/ri/14935
Data de publicação: Ago-2015
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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