Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/14966
Tipo do documento: Artigo
Título: Proteomic profile response of Paracoccidioides lutzii to the antifungal argentilactone
Autor: Prado, Renata Silva do
Bailão, Alexandre Melo
Silva, Lívia do Carmo
Oliveira, Cecília Maria Alves de
Marques, Monique Ferreira
Silva, Luciano Paulino da
Lacerda, Elisângela de Paula Silveira
Lima, Aliny Pereira
Soares, Célia Maria de Almeida
Pereira, Maristela
Abstract: The dimorphic fungi Paracoccidioides spp. are the etiological agents of paracoccidioidomycosis (PCM), a mycosis of high incidence in Brazil. The toxicity of drug treatment and the emergence of resistant organisms have led to research for new candidates for drugs. In this study, we demonstrate that the natural product argentilactone was not cytotoxic or genotoxic to MRCS cells at the 1050 concentration to the fungus. We also verified the proteomic profile of Paracoccidioides lutzii after incubation with argentilactone using a label free quantitative proteome nanoUPLC-MSE. The results of this study indicated that the fungus has a global metabolic adaptation in the presence of argentilactone. Enzymes of important pathways, such as glycolysis, the Krebs cycle and the glyoxylate cycle, were repressed, which drove the metabolism to the methylcytrate cycle and beta-oxidation. Proteins involved in cell rescue, defense and stress response were induced. In this study, alternative metabolic pathways adopted by the fungi were elucidated, helping to elucidate the course of action of the compound studied..
Palavras-chave: Antifungal
Paracoccidioides lutzzi
Paracoccidioidomycosis
Proteomic
Argentilactone
País: Suica
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: PRADO, Renata Silva do et al. Proteomic profile response of Paracoccidioides lutzii to the antifungal argentilactone. Frontiers in Microbiology, Lausanne, v. 6, n. 616, p.1-14, Jan. 2015.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.3389/fmicb.2015.00616
Identificador do documento: 10.3389/fmicb.2015.00616
URI: http://repositorio.bc.ufg.br/handle/ri/14966
Data de publicação: Jan-2015
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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