Transcriptome profile of the response of Paracoccidioides spp. to a camphene thiosemicarbazide derivative
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2015
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Paracoccidioidomycosis (PCM) is a systemic granulomatous human mycosis caused by
fungi of the genus Paracoccidioides, which is geographically restricted to Latin America. Inhalation
of spores, the infectious particles of the fungus, is a common route of infection. The
PCM treatment of choice is azoles such as itraconazole, but sulfonamides and amphotericin
B are used in some cases despite their toxicity tomammalian cells. The current availability of
treatments highlights the need to identify and characterize novel targets for antifungal treatment
of PCMas well as the need to search for new antifungal compounds obtained fromnatural
sources or by chemical synthesis. To this end, we evaluated the antifungal activity of a
camphene thiosemicarbazide derivative (TSC-C) compound on Paracoccidioides yeast. To
determine the response of Paracoccidioides spp. to TSC-C, we analyzed the transcriptional
profile of the fungus after 8 h of contact with the compound. The results demonstrate that
Paracoccidioides lutzii induced the expression of genes related to metabolism; cell cycle and
DNA processing; biogenesis of cellular components; cell transduction/signal; cell rescue,
defense and virulence; cellular transport, transport facilities and transport routes; energy; protein
synthesis; protein fate; transcription; and other proteins without classification. Additionally,
we observed intensely inhibited genes related to protein synthesis. Analysis by
fluorescence microscopy and flow cytometry revealed that the compound induced the production
of reactive oxygen species. Using an isolate with down-regulated SOD1 gene expression
(SOD1-aRNA), we sought to determine the function of this gene in the defense of
Paracoccidioides yeast cells against the compound. Mutant cells were more susceptible to
TSC-C, demonstrating the importance of this gene in response to the compound. The results
presented herein suggest that TSC-C is a promising candidate for PCM treatment.
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SILVA, Lívia do Carmo Silva et al. Transcriptome profile of the response of Paracoccidioides spp. To a camphene thiosemicarbazide derivative. Plos One, San Francisco, v. 10, n. 6, e0130703, 2015.