Use este identificador para citar ou linkar para este item:
Tipo do documento: Artigo
Título: Transcriptome profile of the response of Paracoccidioides spp. to a camphene thiosemicarbazide derivative
Autor: Silva, Lívia do Carmo
Ossa, Diana Patrícia Tamayo
Castro, Symone Vitoriano da Conceição
Pires, Ludmila Bringel
Oliveira, Cecília Maria Alves de
Silva, Cleuza Conceição da
Coelho, Narcimário Pereira
Bailão, Alexandre Melo
Rocha, Juliana Alves Parente
Soares, Célia Maria de Almeida
Ruiz, Orville Hernández
Ochoa, Juan Guillermo McEwen
Pereira, Maristela
Abstract: Paracoccidioidomycosis (PCM) is a systemic granulomatous human mycosis caused by fungi of the genus Paracoccidioides, which is geographically restricted to Latin America. Inhalation of spores, the infectious particles of the fungus, is a common route of infection. The PCM treatment of choice is azoles such as itraconazole, but sulfonamides and amphotericin B are used in some cases despite their toxicity tomammalian cells. The current availability of treatments highlights the need to identify and characterize novel targets for antifungal treatment of PCMas well as the need to search for new antifungal compounds obtained fromnatural sources or by chemical synthesis. To this end, we evaluated the antifungal activity of a camphene thiosemicarbazide derivative (TSC-C) compound on Paracoccidioides yeast. To determine the response of Paracoccidioides spp. to TSC-C, we analyzed the transcriptional profile of the fungus after 8 h of contact with the compound. The results demonstrate that Paracoccidioides lutzii induced the expression of genes related to metabolism; cell cycle and DNA processing; biogenesis of cellular components; cell transduction/signal; cell rescue, defense and virulence; cellular transport, transport facilities and transport routes; energy; protein synthesis; protein fate; transcription; and other proteins without classification. Additionally, we observed intensely inhibited genes related to protein synthesis. Analysis by fluorescence microscopy and flow cytometry revealed that the compound induced the production of reactive oxygen species. Using an isolate with down-regulated SOD1 gene expression (SOD1-aRNA), we sought to determine the function of this gene in the defense of Paracoccidioides yeast cells against the compound. Mutant cells were more susceptible to TSC-C, demonstrating the importance of this gene in response to the compound. The results presented herein suggest that TSC-C is a promising candidate for PCM treatment.
País: Estados unidos
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: SILVA, Lívia do Carmo Silva et al. Transcriptome profile of the response of Paracoccidioides spp. To a camphene thiosemicarbazide derivative. Plos One, San Francisco, v. 10, n. 6, e0130703, 2015.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1371/journal.pone.0130703
Identificador do documento: 10.1371/journal.pone.0130703
Data de publicação: 2015
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Artigo - Lívia do Carmo Silva - 2015.pdf13,66 MBAdobe PDFThumbnail

Este item está licenciada sob uma Licença Creative Commons Creative Commons