Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/14979
Tipo do documento: Artigo
Título: Analysis of the secretomes of paracoccidioides mycelia and yeast cells
Autor: Weber, Simone Schneider
Parente, Ana Flávia Alves
Borges, Clayton Luiz
Parente, Juliana Alves
Bailão, Alexandre Melo
Soares, Célia Maria de Almeida
Abstract: Paracoccidioides, a complex of several phylogenetic species, is the causative agent of paracoccidioidomycosis. The ability of pathogenic fungi to develop a multifaceted response to the wide variety of stressors found in the host environment is important for virulence and pathogenesis. Extracellular proteins represent key mediators of the host-parasite interaction. To analyze the expression profile of the proteins secreted by Paracoccidioides, Pb01 mycelia and yeast cells, we used a proteomics approach combining two-dimensional electrophoresis with matrix-assisted laser desorption ionization quadrupole time-of-flight mass spectrometry (MALDI-Q-TOF MS/MS). From three biological replicates, 356 and 388 spots were detected, in mycelium and yeast cell secretomes, respectively. In this study, 160 non-redundant proteins/isoforms were indentified, including 30 and 24 proteins preferentially secreted in mycelia and yeast cells, respectively. In silico analyses revealed that 65% of the identified proteins/isoforms were secreted primarily via non-conventional pathways. We also investigated the influence of protein export inhibition in the phagocytosis of Paracoccidioides by macrophages. The addition of Brefeldin A to the culture medium significantly decreased the production of secreted proteins by both Paracoccidioides and internalized yeast cells by macrophages. In contrast, the addition of concentrated culture supernatant to the co-cultivation significantly increased the number of internalized yeast cells by macrophages. Importantly, the proteins detected in the fungal secretome were also identified within macrophages. These results indicate that Paracoccidioides extracellular proteins are important for the fungal interaction with the host.
País: Estados unidos
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: WEBER, Simone Schneider et al. Analysis of the secretomes of paracoccidioides mycelia and yeast cells. Plos One, San Francisco, v. 7, n. 12, e52470, 2012.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1371/journal.pone.0052470
Identificador do documento: 10.1371/journal.pone.0052470
URI: http://repositorio.bc.ufg.br/handle/ri/14979
Data de publicação: 2012
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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