Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15091
Tipo do documento: Artigo
Título: Predicting copper-, iron-, and zinc-binding proteins in pathogenic species of the Paracoccidioides genus
Autor: Tristão, Gabriel Brum
Assunção, Leandro do Prado
Santos, Luiz Paulo Araújo dos
Borges, Clayton Luiz
Silva-Bailão, Mirelle Garcia
Soares, Célia Maria de Almeida
Cavallaro, Gabriele
Bailão, Alexandre de Melo
Abstract: Approximately one-third of all proteins have been estimated to contain at least one metal cofactor, and these proteins are referred to as metalloproteins. These represent one of the most diverse classes of proteins, containing metal ions that bind to specific sites to perform catalytic, regulatory and structural functions. Bioinformatic tools have been developed to predict metalloproteins encoded by an organism based only on its genome sequence. Its function and the type of metal binder can also be predicted via a bioinformatics approach. Paracoccidioides complex includes termodimorphic pathogenic fungi that are found as saprobic mycelia in the environment and as yeast, the parasitic form, in host tissues. They are the etiologic agents of Paracoccidioidomycosis, a prevalent systemic mycosis in Latin America. Many metalloproteins are important for the virulence of several pathogenic microorganisms. Accordingly, the present work aimed to predict the copper, iron and zinc proteins encoded by the genomes of three phylogenetic species of Paracoccidioides (Pb01, Pb03, and Pb18). The metalloproteins were identified using bioinformatics approaches based on structure, annotation and domains. Cu-, Fe-, and Zn-binding proteins represent 7% of the total proteins encoded by Paracoccidioides spp. genomes. Zinc proteins were the most abundant metalloproteins, representing 5.7% of the fungus proteome, whereas copper and iron proteins represent 0.3 and 1.2%, respectively. Functional classification revealed that metalloproteins are related to many cellular processes. Furthermore, it was observed that many of these metalloproteins serve as virulence factors in the biology of the fungus. Thus, it is concluded that the Cu, Fe, and Zn metalloproteomes of the Paracoccidioides spp. are of the utmost importance for the biology and virulence of these particular human pathogens.
Palavras-chave: Metalloproteome
Virulence
Bioinformatics
Paracoccidioidomycosis
Metalhomeostasis
País: Suica
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: TRISTÂO, Gabriel B. et al. Predicting copper-, iron-, and zinc-binding proteins in pathogenic species of the Paracoccidioides genus. Frontiers in Microbiology, Lausanne, v. 5, n. 761, p. 1-11, jan. 2015.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.3389/fmicb.2014.00761
Identificador do documento: 10.3389/fmicb.2014.00761
URI: http://repositorio.bc.ufg.br/handle/ri/15091
Data de publicação: Jan-2015
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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