Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15093
Tipo do documento: Artigo
Título: A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteins
Autor: Parente, Juliana Alves
Furlaneto, Silvia Maria Salem Izacc
Santana, Jaime Martins de
Pereira, Maristela
Borges, Clayton Luiz
Bailão, Alexandre Melo
Soares, Célia Maria de Almeida
Abstract: Background: Paracoccidioides brasiliensis is a thermodimorphic fungus, the causative agent of paracoccidioidomycosis (PCM). Serine proteases are widely distributed and this class of peptidase has been related to pathogenesis and nitrogen starvation in pathogenic fungi. Results: A cDNA (Pbsp) encoding a secreted serine protease (PbSP), was isolated from a cDNA library constructed with RNAs of fungal yeast cells recovered from liver of infected mice. Recombinant PbSP was produced in Escherichia coli, and used to develop polyclonal antibodies that were able to detect a 66 kDa protein in the P. brasiliensis proteome. In vitro deglycosylation assays with endoglycosidase H demonstrated that PbSP is a N-glycosylated molecule. The Pbsp transcript and the protein were induced during nitrogen starvation. The Pbsp transcript was also induced in yeast cells infecting murine macrophages. Interactions of PbSP with P. brasiliensis proteins were evaluated by two-hybrid assay in the yeast Saccharomyces cerevisiae. PbSP interacts with a peptidyl prolyl cis-trans isomerase, calnexin, HSP70 and a cell wall protein PWP2. Conclusions: A secreted subtilisin induced during nitrogen starvation was characterized indicating the possible role of this protein in the nitrogen acquisition. PbSP interactions with other P. brasiliensis proteins were reported. Proteins interacting with PbSP are related to folding process, protein trafficking and cytoskeleton reorganization.
País: Gra-bretanha
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: PARENTE, Juliana A. et al. A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteins. BMC Microbiology, London, v. 10, n. 292, 2010.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1186/1471-2180-10-292
Identificador do documento: 10.1186/1471-2180-10-292
URI: http://repositorio.bc.ufg.br/handle/ri/15093
Data de publicação: 2010
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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