Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15181
Tipo do documento: Artigo
Título: The characterization of a new set of EST-derived simple sequence repeat (SSR) markers as a resource for the genetic analysis of Phaseolus vulgaris
Autor: Garcia, Robertha Augusta Vasconcelos
Rangel, Priscila Nascimento
Brondani, Claudio
Martins, Wellington Santos
Melo, Leonardo Cunha
Carneiro, Monalisa Sampaio
Borba, Tereza Cristina Oliveira
Brondani, Rosana Pereira Vianelo
Abstract: Background: Over recent years, a growing effort has been made to develop microsatellite markers for the genomic analysis of the common bean (Phaseolus vulgaris) to broaden the knowledge of the molecular genetic basis of this species. The availability of large sets of expressed sequence tags (ESTs) in public databases has given rise to an expedient approach for the identification of SSRs (Simple Sequence Repeats), specifically EST-derived SSRs. In the present work, a battery of new microsatellite markers was obtained from a search of the Phaseolus vulgaris EST database. The diversity, degree of transferability and polymorphism of these markers were tested. Results: From 9,583 valid ESTs, 4,764 had microsatellite motifs, from which 377 were used to design primers, and 302 (80.11%) showed good amplification quality. To analyze transferability, a group of 167 SSRs were tested, and the results showed that they were 82% transferable across at least one species. The highest amplification rates were observed between the species from the Phaseolus (63.7%), Vigna (25.9%), Glycine (19.8%), Medicago (10.2%), Dipterix (6%) and Arachis (1.8%) genera. The average PIC (Polymorphism Information Content) varied from 0.53 for genomic SSRs to 0.47 for EST-SSRs, and the average number of alleles per locus was 4 and 3, respectively. Among the 315 newly tested SSRs in the BJ (BAT93 X Jalo EEP558) population, 24% (76) were polymorphic. The integration of these segregant loci into a framework map composed of 123 previously obtained SSR markers yielded a total of 199 segregant loci, of which 182 (91.5%) were mapped to 14 linkage groups, resulting in a map length of 1,157 cM. Conclusions: A total of 302 newly developed EST-SSR markers, showing good amplification quality, are available for the genetic analysis of Phaseolus vulgaris. These markers showed satisfactory rates of transferability, especially between species that have great economic and genomic values. Their diversity was comparable to genomic SSRs, and they were incorporated in the common bean reference genetic map, which constitutes an important contribution to and advance in Phaseolus vulgaris genomic research.
País: Outros
Unidade acadêmica: Instituto de Informática - INF (RG)
Citação: GARCIA, Robertha A. V.; RANGEL, Priscila N.; BRONDANI, Claudio; MARTINS, Wellington S.; MELO, Leonardo C.; CARNEIRO, Monalisa S.; BORBA, Tereza C. O.; BRONDANI, Rosana P. V. The characterization of a new set of EST-derived simple sequence repeat (SSR) markers as a resource for the genetic analysis of Phaseolus vulgaris. BMC Genetics, London, v. 12, n. 41, p. 1-14, 2011.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1186/1471-2156-12-41
Identificador do documento: 10.1186/1471-2156-12-41
URI: http://repositorio.bc.ufg.br/handle/ri/15181
Data de publicação: 2011
Aparece nas coleções:INF - Artigos publicados em periódicos

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