Transcriptional profile of Paracoccidioides induced by oenothein B, a potential antifungal agent from the Brazilian Cerrado plant Eugenia uniflora
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2013
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Background: The compound oenothein B (OenB), which is isolated from the leaves of Eugenia uniflora, a Brazilian
Cerrado plant, interferes with Paracoccidioides yeast cell morphology and inhibits 1,3-β-D-glucan synthase (PbFKS1)
transcript accumulation, which is involved in cell wall synthesis. In this work we examined the gene expression
changes in Paracoccidioides yeast cells following OenB treatment in order to investigate the adaptive cellular
responses to drug stress.
Results: We constructed differential gene expression libraries using Representational Difference Analysis (RDA) of
Paracoccidioides yeast cells treated with OenB for 90 and 180 min. Treatment for 90 min resulted in the
identification of 463 up-regulated expressed sequences tags (ESTs) and 104 down-regulated ESTs. For the 180 min
treatment 301 up-regulated ESTs and 143 down-regulated were identified. Genes involved in the cell wall
biosynthesis, such as GLN1, KRE6 and FKS1, were found to be regulated by OenB. Infection experiments in
macrophages corroborated the in vitro results. Fluorescence microscopy showed increased levels of chitin in cells
treated with OenB. The carbohydrate polymer content of the cell wall of the fungus was also evaluated, and the
results corroborated with the transcriptional data. Several other genes, such as those involved in a variety of
important cellular processes (i.e., membrane maintenance, stress and virulence) were found to be up-regulated in
response to OenB treatment.
Conclusions: The exposure of Paracoccidioides to OenB resulted in a complex altered gene expression profile.
Some of the changes may represent specific adaptive responses to this compound in this important pathogenic
fungus.
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Paracoccidioides, Antifungal, Oenothein B, Transcriptome, Cell wall
Citação
CARVALHO, Patrícia Fernanda Zambuzzi; TOMAZETT, Patrícia Kott; SANTOS, Suzana Costa; Ferri, Pedro Henrique; BORGES, Clayton Luiz; MARTINS, Wellington Santos; SOARES, Célia Maria; PEREIRA, Maristela. Transcriptional profile of Paracoccidioides induced by oenothein B, a potential antifungal agent from the Brazilian Cerrado plant Eugenia uniflora. BMC Microbiology, Bethesda, v. 13, n. 227, p. 1-16, 2013.