Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15231
Tipo do documento: Artigo
Título: sodC-based real-time PCR for detection of neisseria meningitidis
Autor: Thomas, Jennifer Dolan
Hatcher, Cynthia P.
Satterfield, Dara A.
Theodore, M. Jordan
Bach, Michelle C.
Linscott, Kristin B.
Zhao, Xin
Wang, Xin
Mair, Raydel
Schmink, Susanna
Arnold, Kathryn E.
Stephens, David S.
Harrison, Lee H.
Hollick, Rosemary A.
Andrade, Ana Lúcia Sampaio Sgambatti de
Cardoso, Juliana Lamaro
Lemos, Ana Paula Silva
Gritzfeld, Jenna
Gordon, Stephen
Soysal, Ahmet
Bakir, Mustafa
Sharma, Dolly
Jain, Shabnam
Satola, Sarah W.
Messonnier, Nancy E.
Mayer, Leonard W.
Abstract: Real-time PCR (rt-PCR) is a widely used molecular method for detection of Neisseria meningitidis (Nm). Several rt-PCR assays for Nm target the capsule transport gene, ctrA. However, over 16% of meningococcal carriage isolates lack ctrA, rendering this target gene ineffective at identification of this sub-population of meningococcal isolates. The Cu-Zn superoxide dismutase gene, sodC, is found in Nm but not in other Neisseria species. To better identify Nm, regardless of capsule genotype or expression status, a sodC-based TaqMan rt-PCR assay was developed and validated. Standard curves revealed an average lower limit of detection of 73 genomes per reaction at cycle threshold (Ct) value of 35, with 100% average reaction efficiency and an average R2 of 0.9925. 99.7% (624/626) of Nm isolates tested were sodC-positive, with a range of average Ct values from 13.0 to 29.5. The mean sodC Ct value of these Nm isolates was 17.662.2 (6SD). Of the 626 Nm tested, 178 were nongroupable (NG) ctrA-negative Nm isolates, and 98.9% (176/178) of these were detected by sodC rt-PCR. The assay was 100% specific, with all 244 non-Nm isolates testing negative. Of 157 clinical specimens tested, sodC detected 25/157 Nm or 4 additional specimens compared to ctrA and 24 more than culture. Among 582 carriage specimens, sodC detected Nm in 1 more than ctrA and in 4 more than culture. This sodC rt-PCR assay is a highly sensitive and specific method for detection of Nm, especially in carriage studies where many meningococcal isolates lack capsule genes.
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: THOMAS, Jennifer Dolan et al. sodC-based real-time PCR for detection of neisseria meningitidis. Plos One, San Francisco, v. 6, n. 5, e19361, 2011.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1371/journal.pone.0019361
Identificador do documento: 10.1371/journal.pone.0019361
URI: http://repositorio.bc.ufg.br/handle/ri/15231
Data de publicação: 2011
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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