Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15235
Tipo do documento: Artigo
Título: Molecular epidemiology of coagulase-negative Staphylococcus carriage in neonates admitted to an intensive care unit in Brazil
Autor: Ternes, Yves Mauro
Cardoso, Juliana Lamaro
André, Maria Cláudia Porfirio
Pessoa Júnior, Vicente Porfírio
Vieira, Maria Aparecida da Silva
Minamisava, Ruth
Andrade, Ana Lúcia Sampaio Sgambatti de
Kipnis, André
Abstract: Background: Nasal colonization with coagulase-negative Staphylococcus (CoNS) has been described as a risk factor for subsequent systemic infection. In this study, we evaluated the genetic profile of CoNS isolates colonizing the nares of children admitted to a neonatal intensive care unit (NICU). Methods: We assessed CoNS carriage at admittance and discharge among newborns admitted to a NICU from July 2007 through May 2008 in one of the major municipalities of Brazil. Isolates were screened on mannitol salt agar and tryptic soy broth and tested for susceptibility to antimicrobials using the disc diffusion method. Polymerase chain reaction (PCR) was used to determine the species, the presence of the mecA gene, and to perform SCCmec typing. S. epidermidis and S. haemolyticus isolated from the same child at both admission and discharge were characterized by PFGE. Results: Among 429 neonates admitted to the NICU, 392 (91.4%) had nasal swabs collected at both admission and discharge. The incidence of CoNS during the hospitalization period was 55.9% (95% confidence interval [CI]: 50.9- 60.7). The most frequently isolated species were S. haemolyticus (38.3%) and S.epidermidis (38.0%). Multidrug resistance (MDR) was detected in 2.2% and 29.9% of the CoNS isolates, respectively at admittance and discharge (p = 0.053). The mecA gene was more prevalent among strains isolated at discharge (83.6%) than those isolated at admission (60%); overall, SCCmec type I was isolated most frequently. The length of hospitalization was associated with colonization by MDR isolates (p < 0.005). Great genetic diversity was observed among S. epidermidis and S. haemolyticus. Conclusions: NICU represents an environment of risk for colonization by MDR CoNS. Neonates admitted to the NICU can become a reservoir of CoNS strains with the potential to spread MDR strains into the community.
Palavras-chave: mecA
Coagulase-negative Staphylococcus
Neonates
SCCmec
Neonatal intensive care units
País: Estados unidos
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: TERNES, Yves Mauro et. al. Molecular epidemiology of coagulase-negative Staphylococcus carriage in neonates admitted to an intensive care unit in Brazil. BMC Infectious Diseases, London, v. 13, n. 572, 2013.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1186/1471-2334-13-572
Identificador do documento: 10.1186/1471-2334-13-572
URI: http://repositorio.bc.ufg.br/handle/ri/15235
Data de publicação: 2013
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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