Trypanocidal action of (−)-elatol involves an oxidative stress triggered by Mitochondria dysfunction
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Data
2012
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Resumo
Natural compounds have shown good potential for the discovery of new
chemotherapeutics for the treatment of Chagas’ disease. Recently, our group reported
the effective trypanocidal activity of (−)-elatol, extracted from the red macroalgae
Laurencia dendroidea present in the Brazilian coast against Trypanosoma cruzi. However,
the mechanism of action of this compound has remained unclear. There are only hypotheses
concerning its action on mitochondrial function. Here, we further investigated the mechanisms
of action of (−)-elatol on trypomastigotes of T. cruzi. For this, we evaluated some
biochemical alterations in trypomastigotes treated with (−)-elatol. Our results show that
(−)-elatol induced depolarization of the mitochondrial membrane, an increase in the
formation of mitochondrial superoxide anion and loss of cell membrane and DNA integrity.
Additionally, (−)-elatol induced formation of autophagic vacuoles and a decrease in cell
volume. All together, these results suggest that the trypanocidal action of (−)-elatol involves multiple events and mitochondria might be the initial target organelle. Our hypothesis is
that the mitochondrial dysfunction leads to an increase of ROS production through the
electron transport chain, which affects cell membrane and DNA integrity leading to
different types of parasite death.
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(−)-elatol, Trypanosoma cruzi, Chagas disease, Mitochondria, Oxidative stress
Citação
DESOTI, Vânia Cristina; LAZARIN, Danielle Bidoia; SUDATTI, Daniela Bueno; PEREIRA, Renato Crespo; ALONSO, Antonio; NAKAMURA, Tania Ueda; DIAS FILHO, Benedito Prado; NAKAMURA, Celso Vataru; OLIVEIRA Silva, Sueli de. Trypanocidal action of (−)-elatol involves an oxidative stress triggered by Mitochondria dysfunction. Marine Drugs, Basel, v. 10, p. 1631-1646, 2012.