Clinical and microbiological features of cryptococcal meningitis
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2013-06
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In this study, the clinical features, underlying diseases and clinical outcomes of patients with cryptococcosis
were investigated. In addition, a molecular analysis of the Cryptococcus neoformans species complex isolated from these
patients was performed. Methods: A prospective study of 62 cases of patients with cryptococcal infection was conducted at
the Hospital de Doenças Tropicais de Goiás Dr. Anuar Auad from 2009-2010. Cryptococcal meningitis cases were diagnosed
by direct examination and cerebrospinal fl uid (CSF) sample culture. The profi ling of these patients was assessed. The CSF
samples were submitted to India ink preparation and cultured on Sabouraud dextrose agar, and C. neoformans was identifi ed by
the production of urease, a positive phenoloxidase test and assimilation of carbohydrates. C. neoformans and C. gattii isolates
were distinguished by growth on L-canavanine-glycine-bromothymol blue medium, and molecular analysis was conducted via
PCR fi ngerprinting reactions using M13 and (GACA) 4 primers. Results: From the 62 patients with cryptococcosis, 71 isolates
of CSF were obtained; 67 (94.4%) isolates were identifi ed as C. neoformans var. grubii/VNI, and 4 (5.6%) were identifi ed as
C. gattii/VGII. Of these patients, 53 had an HIV diagnosis. The incidence of cryptococcosis was higher among patients 20-40
years of age, with 74.2% of the cases reported in males. Cryptococcus-related mortality was noted in 48.4% of the patients, and
the symptoms were altered sensorium, headache, fever and stiff neck. Conclusions: The high morbidity and mortality observed
among patients with cryptococcosis demonstrate the importance of obtaining information regarding the epidemiological profi le
and clinical course of the disease in the State of Goiás, Brazil.
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Cryptococcosis, HIV, Epidemiological and clinical features.
Citação
SOUZA, Lúcia Kioko Hasimoto e; COSTA, Carolina Rodrigues; FERNANDES, Orionalda de Fátima Lisboa; ABRÃO, Fernando Yano; SILVA, Thaisa Cristina; TREMÉA, Carolina Martins; SILVA, Maria do Rosário Rodrigues. Clinical and microbiological features of cryptococcal meningitis. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 46, n. 3, p. 343-347, May/June 2013.