Differences in exoenzymes production and adherence ability among Candida spp. isolates from catheter, blood and oral cavity
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Data
2010-06
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Resumo
Phospholipase and proteinase production and the ability of adhesion to buccal epithelial cells (BEC) of 112 Candida isolates
originated from oral cavity of HIV infected patients and from blood and catheter of intensive care unit patients were investigated.
The proteinase production was detected by inoculation into bovine serum albumin (BSA) agar and the phospholipase activity was
performed using egg yolk emulsion. A yeast suspension of each test strain was incubated with buccal epithelial cells and the number
of adherence yeast to epithelial cells was counted. A percentage of 88.1% and 55.9% of Candida albicans and 69.8% and 37.7% of
non-albicans Candida isolates produced proteinase and phospholipase, respectively. Non-albicans Candida isolated from catheter were
more proteolytic than C. albicans isolates. Blood isolates were more proteolytic than catheter and oral cavity isolates while oral cavity
isolates produced more phospholipase than those from blood and catheter. C. albicans isolates from oral cavity and from catheter were
more adherent to BEC than non-albicans Candida isolates, but the adhesion was not different among the three sources analyzed. The
results indicated differences in the production of phospholipase and proteinase and in the ability of adhesion to BEC among Candida
spp. isolates from different sources. This study suggests that the pathogenicity of Candida can be correlated with the infected site.
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Adherence ability, Exoenzyme activity, Candida
Citação
COSTA, Carolina Rodrigues; PASSOS, Xisto Sena; SOUZA, Lúcia Kioko Hasimoto e; LUCENA, Percilia de Andrade; FERNANDES, Orionalda de Fátima Lisboa; SILVA, Maria do Rosário Rodrigues. Differences in exoenzymes production and adherence ability among Candida spp. isolates from catheter, blood and oral cavity. Revista do Instituto de Medicina Tropical de São Paulo, São Paulo, v. 52, n. 3, p. 139-143, May/June 2010.