Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15340
Tipo do documento: Artigo
Título: Antifungal and cytotoxicity activities of the fresh xylem sap of Hymenaea courbaril L. and its major constituent fisetin
Autor: Costa, Maysa Paula da
Valadares, Marize Campos
Andrade, Wanessa Machado
Costa, Carolina Rodrigues
Silva, Alessandro Lopes da
Oliveira, Cecilia Maria Alves de
Kato, Lucilia
Fernandes, Orionalda de Fátima Lisboa
Souza, Lúcia Kioko Hasimoto e
Silva, Maria do Rosário Rodrigues
Abstract: Background: The great potential of plants as Hymenaea courbaril L (jatoba) has not yet been throughly explored scientifically and therefore it is very important to investigate their pharmacological and toxicological activities to establish their real efficacy and safety. This study investigated the cytotoxicity of xylem sap of Hymenaea courbaril L and its bioactivity against the fungi Cryptococcus neoformans species complex and dermatophytes. Methods: The fresh xylem sap of H. courbaril was filtered resulting in an insoluble brown color precipitate and was identified as fisetin. In the filtrate was identified the mixture of fisetinediol, fustin, 3-O-methyl-2,3-trans-fustin and taxifolin, which were evaluated by broth microdilution antifungal susceptibility testing against C. neoformans species complex and dermatophytes. The fresh xylem sap and fisetin were screened for cytotoxicity against the 3T3-A31 cells of Balb/c using neutral red uptake (NRU) assay. Results: The fresh xylem sap and the fisetin showed higher in vitro activity than the filtrate. The xylem sap of H. courbaril inhibited the growth of dermatophytes and of C. neoformans with minimal inhibition concentration (MIC) < 256 μg/mL, while the fisetin showed MIC < 128 μg/mL for these fungi. Fisetin showed lower toxicity (IC 50 = 158 μg/mL) than the fresh xylem sap (IC 50 = 109 μg/mL). Conclusion: Naturally occurring fisetin can provide excellent starting points for clinical application and can certainly represent a therapeutic potential against fungal infections, because it showed in vitro antifungal activity and low toxicity on animal cells.
Palavras-chave: Antifungal activity
Cytotoxicity
Hymenaea courbaril
País: Gra-bretanha
Unidade acadêmica: Instituto de Patologia Tropical e Saúde Pública - IPTSP (RG)
Citação: COSTA, Maysa Paula da; BOZINIS, Marize Campos Valadares; ANDRADE, Wanessa Machado; COSTA, Carolina Rodrigues; SILVA, Alessandro Lopes da;OLIVEIRA, Cecília Maria Alves de; KATO, Lucília; FERNANDES, Orionalda de Fátima Lisboa ; SOUZA, Lúcia Kioko Hasimoto; SILVA, Maria Do Rosário Rodrigues. Antifungal and cytotoxicity activities of the fresh xylem sap of Hymenaea courbaril L. and its major constituent fisetin. BMC Complementary and Alternative Medicine, London, v. 14, e245, 2014.
Tipo de acesso: Acesso Aberto
URI: http://repositorio.bc.ufg.br/handle/ri/15340
Data de publicação: 2014
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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