Use este identificador para citar ou linkar para este item: http://repositorio.bc.ufg.br/handle/ri/15345
Tipo do documento: Artigo
Título: Molecular characterization of the NSP4 gene of human group A rotavirus samples from the west central region of Brazil
Autor: Miranda, Talissa de Moraes Tavares
Brito, Wilia Marta Elsner Diederichsen de
Fiaccadori, Fabíola Souza
Freitas, Erika Regina Leal de
Rocha, Juliana Alves Parente
Costa, Paulo Sérgio Sucasas da
Giugliano, Loreny Gimenes
Andreasi, Márcia Sueli Assis
Soares, Célia Maria Almeida
Cardoso, Divina das Dôres de Paula
Abstract: Nonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavi- rus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveil- lance of rotavirus infections and vaccine development.
Palavras-chave: Group A rotavirus
West central region
NSP4 gene
Genotypes
Brazil
País: Brasil
Unidade acadêmica: Instituto de Ciências Biológicas - ICB (RG)
Citação: TAVARES, Talissa de Moraes; BRITO, Wilia Marta Elsner Diederichsen de; FIACCADORI, Fabíola Souza; FREITAS, Erika Regina Leal de; PARENTE, Juliana Alves ; COSTA, Paulo Sérgio Sucasas da; GUIGLIANO, Loreny Gimenes; ANDREASI, Márcia Sueli Assis; SOARES, Célia Maria Almeida; CARDOSO, Divina das Dores de Paula. Molecular characterization of the NSP4 gene of human group A rotavirus samples from the west central region of Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 3, p. 288-294, May 2008.
Tipo de acesso: Acesso Aberto
Identificador do documento: 10.1590/S0074-02762008000300011
Identificador do documento: 10.1590/S0074-02762008000300011
URI: http://repositorio.bc.ufg.br/handle/ri/15345
Data de publicação: Mai-2008
Aparece nas coleções:IPTSP - Artigos publicados em periódicos

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